Chojecka Agnieszka, Tarka Patryk, Kierzkowska Anna, Nitsch-Osuch Aneta, Kanecki Krzysztof
National Institute of Public Health – National Institute of Hygiene, Department of Bacteriology, Warsaw, Poland
Medical University of Warsaw, Department of Social Medicine and Public Health, Warsaw, Poland
Rocz Panstw Zakl Hig. 2017;68(4):389-394.
Alcohols are the most commonly used active substances in preparations for quick hand disinfection. They should be bactericidal in very short contact time. PN-EN 13727 + A2: 2015-12 standard, for testing hygienic and surgical handrub disinfection preparations, provides mandatory test conditions of disinfectants in contact times with the range of 30 s to 60 s (hygienic handrub disinfection) and 60 s to 5 min (surgical handrub disinfection). A short contact times for hand hygiene products require a short time of neutralization process. For contact times less than or equal to 10 minutes, the estimated neutralization time is 10 s ± 1 s. Neutralization is a process that abolishes the action of disinfectants. Correct application of this process allows for proper use of disinfectants in practice and its biocidal effect. Objectives. Verification of the effectiveness of 10-second neutralization time of alcohol based preparations for hygienic handrub disinfection
Neutralization of two products with different ethanol content (89% and 70%) for hygienic handrub disinfection according to PN-EN 13727 + A2: 2015-12 was investigated. The effectiveness of the neutralizer was assessed by determining toxicity of neutralizer, activity of residual effects of the tested products and their derivatives produced during neutralization (10 s) for test organisms (Staphylococcus aureus ATCC 6538; Pseudomonas aeruginosa ATCC 15442; Enterococcus hirae ATCC 10541; Escherichia coli K12 NCTC 10538)
The 10-second neutralization time was sufficient to eliminate the residual activity of products for hygienic handrub disinfection with differentiated ethanol concentration. The neutralizer used did not show toxicity to bacteria and did not produce toxic products with tested preparations after neutralization
Conclusions. The use of 10-second neutralization time allows in a precise way designate the contact times for hygienic handrub disinfection products
酒精是速干手消毒剂制剂中最常用的活性物质。它们应在极短的接触时间内具有杀菌作用。PN-EN 13727 + A2: 2015-12标准用于测试卫生手消毒和外科手消毒制剂，规定了消毒剂在30秒至60秒（卫生手消毒）和60秒至5分钟（外科手消毒）接触时间的强制测试条件。手卫生产品的短接触时间要求中和过程时间较短。对于接触时间小于或等于10分钟的情况，估计中和时间为10秒±1秒。中和是消除消毒剂作用的过程。正确应用此过程可确保消毒剂在实际使用中的正确使用及其杀菌效果。目的。验证用于卫生手消毒的酒精制剂10秒中和时间的有效性
根据PN-EN 13727 + A2: 2015-12研究了两种不同乙醇含量（89%和70%）用于卫生手消毒产品的中和情况。通过测定中和剂的毒性、测试产品及其在中和过程（10秒）中产生的衍生物对测试微生物（金黄色葡萄球菌ATCC 6538；铜绿假单胞菌ATCC 15442；海氏肠球菌ATCC 10541；大肠杆菌K12 NCTC 10538）的残留活性来评估中和剂的有效性
10秒的中和时间足以消除不同乙醇浓度的卫生手消毒产品的残留活性。所用中和剂对细菌无毒性，中和后与测试制剂也不产生有毒产物
结论。使用10秒中和时间能够精确确定卫生手消毒产品的接触时间
