School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 310053, PR China.
School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 310053, PR China; Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, 310007, Zhejiang, PR China.
Regul Toxicol Pharmacol. 2018 Feb;92:315-323. doi: 10.1016/j.yrtph.2017.12.018. Epub 2017 Dec 26.
Lamiophlomis rotata(Benth.) Kudo is widely used in traditional Chinese medicine and its iridoid glycosides extract (IGLR) was the main active ingredient with hemostatic, antinociceptive and anti-inflammatory effects. This study was aimed to evaluate the safety of IGLR using acute and sub-chronic toxicity study methods on Sprague-Dawley rats. In acute toxicity test, IGLR caused slight diarrhea in three dose groups and a decreased of RBC and increased of MCH and Ret (P < .05) were observed in 16 g/kg group. In sub-chronic toxicity study, unscheduled deaths occurred in 1 and 3 rats at 0.40 and 1.00 g/kg groups, respectively. A slight diarrhea was observed in 1.00 g/kg group. Hemolytic anemia was the main toxicity effects of IGLR found in 0.40 and 1.00 g/kg groups, with a significant decrease of RBC, HGB (P < .05) and increase of Ret, MCV, MCH (P < .05) in hematological parameters, a significant decrease of ALT, Crea (P < .05) and increase of TBIL (P < .05) in biochemical parameters, and a significant increase of the percentage of rubricyte, normoblast (P < .05) in bone marrow. Overall, this study found IGLR has a potential toxicity considering with hemolytic anemia and diarrhea to rat. These results provide an important reference for further IGLR-related drug exploration.
黄褐毛忍冬（Benth.）Kudo 在传统中药中被广泛应用，其环烯醚萜苷提取物（IGLR）是主要的活性成分，具有止血、镇痛和抗炎作用。本研究旨在通过对 Sprague-Dawley 大鼠进行急性和亚慢性毒性试验，评估 IGLR 的安全性。在急性毒性试验中，IGLR 在三个剂量组中引起轻微腹泻，并在 16g/kg 组中观察到 RBC 减少、MCH 和 Ret 增加（P<0.05）。在亚慢性毒性研究中，在 0.40 和 1.00g/kg 组中，分别有 1 只和 3 只大鼠出现非计划死亡。1.00g/kg 组大鼠出现轻微腹泻。溶血性贫血是 IGLR 在 0.40 和 1.00g/kg 组中发现的主要毒性作用，血液学参数中 RBC、HGB 显著降低（P<0.05），Ret、MCV、MCH 显著升高（P<0.05），生化参数中 ALT、Crea 显著降低（P<0.05），TBIL 显著升高（P<0.05），骨髓中红细胞、正成红细胞的百分比显著升高（P<0.05）。总之，本研究发现 IGLR 对大鼠具有潜在的毒性，考虑到溶血性贫血和腹泻。这些结果为进一步探索 IGLR 相关药物提供了重要参考。
