Niu Yanbing, Pang Xiaojing, Cheng Yuanyuan, Wang Defu, Guo Shang, Liu Yong
College of Life Sciences, Shanxi Agricultural University, No.01. Mingxian Road, Taigu, 030801 Shanxi Province China.
Institute of Edible-Fungi, Shanxi Academy of Agricultural Sciences, Taiyuan, 030006 China.
Virusdisease. 2017 Sep;28(3):315-319. doi: 10.1007/s13337-017-0388-2. Epub 2017 Jun 29.
With continued expansion of L. cultivation, viral diseases affecting the crop have become more serious in recent years, causing enormous losses in yield and quality. A virus sample was obtained from Wenshui in Shanxi province, China. Double-stranded RNA technology and sequence-independent amplification (SIA) were used to identify the virus that induced L. mosaic disease. SIA and sequencing results showed the presence of watermelon mosaic virus (WMV) in diseased L. leaves. The complete sequence of WMV from the Shanxi isolate (i.e., WMV-WS) was cloned and analyzed for further characterization. The genomic RNA of WMV-WS is 10,040 nucleotides in length and encodes a putative polyprotein of 3218 amino acids. Phylogenetic analysis indicate that all WMV isolates were divided into four groups and WMV-WS isolate belong to Group 4. Further analysis showed that these WMV isolates were not only to a certain degree related to the host, but also related to geographical origin of isolates. Our results provide information for a better understanding of the genetic diversity of WMV isolates infecting L. in Shanxi, China.
随着L.种植面积的不断扩大，近年来影响该作物的病毒病愈发严重，导致产量和品质大幅下降。从中国山西省文水县获取了一份病毒样本。利用双链RNA技术和序列非依赖扩增（SIA）来鉴定引发L.花叶病的病毒。SIA和测序结果表明，患病L.叶片中存在西瓜花叶病毒（WMV）。克隆了来自山西分离株（即WMV-WS）的WMV完整序列，并进行分析以进一步表征。WMV-WS的基因组RNA长度为10,040个核苷酸，编码一个推定的3218个氨基酸的多聚蛋白。系统发育分析表明，所有WMV分离株分为四组，WMV-WS分离株属于第4组。进一步分析表明，这些WMV分离株不仅在一定程度上与宿主有关，还与分离株的地理来源有关。我们的结果为更好地了解中国山西感染L.的WMV分离株的遗传多样性提供了信息。
