Roshal Mikhail, Flores-Montero Juan A, Gao Qi, Koeber Maesa, Wardrope Jessica, Durie Brian G M, Dogan Ahmet, Orfao Alberto, Landgren Ola
Hematopathology Service, Memorial Sloan Kettering Cancer Center, New York, NY.
Cancer Research Institute of Salamanca, Universidad de Salamanca-Instituto de Investigación Biomédica de Salamanca, Salamanca, Spain.
Blood Adv. 2017 May 3;1(12):728-732. doi: 10.1182/bloodadvances.2016003715. eCollection 2017 May 9.
In patients with multiple myeloma, obtaining posttreatment minimal residual disease (MRD) negativity is associated with longer progression-free survival and overall survival. Here, we compared the diagnostic performance of a single 10-color tube with that of a EuroFlow 8-color 2-tube panel for MRD testing. Bone marrow samples from 41 multiple myeloma patients were tested in parallel using the 2 approaches. Compared with the sum of the cells from the EuroFlow two 8-color tubes, the Memorial Sloan Kettering Cancer Center (MSKCC) single 10-color tube had a slight reduction in total cell number with a mean ratio of 0.85 (range, 0.57-1.46; < .05), likely attributable to permeabilization of the cells. Percent of plasma cells showed a high degree of concordance ( = 0.97) as did normal plasma cells ( = 0.96), consistent with no selective plasma cell loss. Importantly, concordant measurement of residual disease burden was seen with abnormal plasma cells ( = 0.97). The overall concordance between the 2 tests was 98%. In 1 case, there was a discrepancy near the limit of detection of both tests in favor of the slightly greater theoretical sensitivity of the EuroFlow 8-color 2-tube panel (analytical sensitivity limit of MSKCC single 10-color tube: 6 cells in 1 million with at least 3 million cell acquisitions; EuroFlow 8-color 2-tube panel: 2 cells in 1 million with the recommended 10 million cell acquisitions).
在多发性骨髓瘤患者中，实现治疗后最小残留病（MRD）阴性与更长的无进展生存期和总生存期相关。在此，我们比较了用于MRD检测的单个10色管与EuroFlow 8色2管检测板的诊断性能。使用这两种方法对41例多发性骨髓瘤患者的骨髓样本进行了平行检测。与EuroFlow两个8色管中的细胞总数相比，纪念斯隆凯特琳癌症中心（MSKCC）的单个10色管的细胞总数略有减少，平均比例为0.85（范围为0.57 - 1.46；P <.05），这可能归因于细胞的通透化。浆细胞百分比显示出高度一致性（κ = 0.97），正常浆细胞也是如此（κ = 0.96），这与没有选择性浆细胞丢失一致。重要的是，异常浆细胞的残留疾病负担测量结果具有一致性（κ = 0.97）。两种检测之间的总体一致性为98%。在1例病例中，两种检测的检测限附近出现了差异，EuroFlow 8色2管检测板的理论灵敏度略高（MSKCC单个10色管的分析灵敏度限：每百万个细胞中有6个细胞，至少采集300万个细胞；EuroFlow 8色2管检测板：每百万个细胞中有2个细胞，推荐采集1000万个细胞）。
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