Jakobsen L K, Trelborg K F, Kingo P S, Hoyer S, Andersson K-E, Djurhuus J C, Norregaard R, Olsen L H
Department of Clinical Medicine, Department of Urology, Aarhus University, Aarhus N, Denmark.
Physiol Res. 2018 May 4;67(2):283-292. doi: 10.33549/physiolres.933545. Epub 2018 Jan 5.
The expression of aquaporins (AQPs) in the fetal porcine urinary tract and its relation to gestational age has not been established. Tissue samples from the renal pelvis, ureter, bladder and urethra were obtained from porcine fetuses. Samples were examined by RT-PCR (AQPs 1-11), QPCR (AQPs positive on RT-PCR), and immunohistochemistry. Bladder samples were additionally examined by Western blotting. RNA was extracted from 76 tissue samples obtained from 19 fetuses. Gestational age was 60 (n=11) or 100 days (n=8). PCR showed that AQP1, 3, 9 and 11 mRNA was expressed in all locations. The expression of AQP3 increased significantly at all four locations with gestational age, whereas AQP11 significantly decreased. AQP1 expression increased in the ureter, bladder and urethra. AQP9 mRNA expression increased in the urethra and bladder, but decreased in the ureter. AQP5 was expressed only in the urethra. Immunohistochemistry showed AQP1 staining in sub-urothelial vessels at all locations. Western blotting analysis confirmed increased AQP1 protein levels in bladder samples during gestation. Expression levels of AQP1, 3, 5, 9 and 11 in the urinary tract change during gestation, and further studies are needed to provide insights into normal and pathophysiological water handling mechanisms in the fetus.
水通道蛋白（AQPs）在胎猪泌尿道中的表达及其与胎龄的关系尚未明确。从猪胎儿获取肾盂、输尿管、膀胱和尿道的组织样本。样本通过逆转录聚合酶链反应（RT-PCR，检测水通道蛋白1 - 11）、定量聚合酶链反应（QPCR，检测RT-PCR呈阳性的水通道蛋白）和免疫组织化学进行检测。膀胱样本还通过蛋白质免疫印迹法进行检测。从19只胎儿获取的76个组织样本中提取RNA。胎龄为60天（n = 11）或100天（n = 8）。PCR结果显示，水通道蛋白1、3、9和11的mRNA在所有部位均有表达。随着胎龄增加，水通道蛋白3在所有四个部位的表达均显著增加，而水通道蛋白11显著减少。水通道蛋白1在输尿管、膀胱和尿道中的表达增加。水通道蛋白9的mRNA在尿道和膀胱中表达增加，但在输尿管中减少。水通道蛋白5仅在尿道中表达。免疫组织化学显示，所有部位的尿路上皮血管下均有AQP1染色。蛋白质免疫印迹分析证实，妊娠期间膀胱样本中AQP1蛋白水平升高。妊娠期间，泌尿道中AQP1、3、5、9和11的表达水平发生变化，需要进一步研究以深入了解胎儿正常和病理生理状态下的水代谢机制。
