• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

缅甸低传播环境下血清学方法估算疟疾本地传播

Estimation on local transmission of malaria by serological approach under low transmission setting in Myanmar.

机构信息

Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea.

Department of Medical Research, Yangon, Myanmar.

出版信息

Malar J. 2018 Jan 5;17(1):6. doi: 10.1186/s12936-017-2170-8.

DOI:10.1186/s12936-017-2170-8
PMID:29304809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5755288/
Abstract

BACKGROUND

As the prevalence of the malaria has been decreasing in many endemic countries including Myanmar, malaria elimination in Greater Mekong Region was targeted not later than 2030. The relevance of molecular and serological tools to identify residual transmission remains to be established in this setting.

METHODS

One-year cohort study was conducted and sera samples were collected in every 3 months with active and passive case detection for clinical malaria episodes by RDT, microscopy and molecular method. The sera were used to detect the malaria antibody against PfMSP1-19, PvAMA1, PvDBPII and PvMSP1-19 by protein microarray.

RESULTS

Among the recruited 1182 participants, there was no RDT positive case for malaria infection although two vivax infections were detected by microscopy in initial collection. Molecular methods detected the asymptomatic cases of 28/1182 (2.37%) in first, 5/894 (0.42%) in second, 12/944 (1.02%) in third, 6/889 (0.51%) in fourth collection, respectively. Seropositivity rates against the PfMSP1-19, PvMSP1-19, PvAMA1 and PvDBPII were 73/270 (27.0%), 85/270 (31.5%), 65/270 (24.1%) and 160/270 (59.3%), respectively. PfMSP1-19 and PvMSP1-19 showed high and stable antigenicity in acute and subacute samples but declining in 1-year history samples. No cross reactivity of PfMSP1-19 and PvMSP1-19 between the two species and higher seropositivity among the asymptomatic carriers were observed. Mapping data indicated serological surveillance can detect the geographical pattern of malaria infection under low transmission setting.

CONCLUSIONS

These findings support that PfMSP1-19 and PvMSP1-19 are suggested for serosurveillance of the malaria especially in low transmission setting for further necessary actions have to be carried out to eliminate the malaria.

摘要

背景

随着包括缅甸在内的许多疟疾流行国家的疟疾发病率持续下降，大湄公河次区域的疟疾消除目标定在 2030 年之前。在这种情况下，分子和血清学工具对于确定残留传播的相关性仍有待确定。

方法

进行了为期一年的队列研究，并通过 RDT、显微镜和分子方法进行主动和被动病例检测，每 3 个月采集一次血清样本，以检测临床疟疾病例。使用蛋白微阵列检测针对 PfMSP1-19、PvAMA1、PvDBPII 和 PvMSP1-19 的疟疾抗体。

结果

在招募的 1182 名参与者中，虽然在初始采集时通过显微镜检测到两例间日疟感染，但没有 RDT 阳性的疟疾病例。分子方法分别在第一次、第二次、第三次和第四次采集时检测到 28/1182（2.37%）、5/894（0.42%）、12/944（1.02%）和 6/889（0.51%）的无症状病例。针对 PfMSP1-19、PvMSP1-19、PvAMA1 和 PvDBPII 的血清阳性率分别为 73/270（27.0%）、85/270（31.5%）、65/270（24.1%）和 160/270（59.3%）。PfMSP1-19 和 PvMSP1-19 在急性和亚急性样本中具有高且稳定的抗原性，但在 1 年历史样本中则下降。在两种物种之间未观察到 PfMSP1-19 和 PvMSP1-19 的交叉反应性，且无症状携带者的血清阳性率更高。绘图数据表明，血清学监测可以在低传播环境下检测疟疾的地理分布模式。

结论

这些发现支持 PfMSP1-19 和 PvMSP1-19 可用于疟疾血清学监测，特别是在低传播环境下，需要进一步采取必要措施消除疟疾。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbc7/5755288/17e8344124db/12936_2017_2170_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbc7/5755288/6fcfa33b1edb/12936_2017_2170_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbc7/5755288/f2352c51e3c0/12936_2017_2170_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbc7/5755288/bc3ba5703842/12936_2017_2170_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbc7/5755288/17e8344124db/12936_2017_2170_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbc7/5755288/6fcfa33b1edb/12936_2017_2170_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbc7/5755288/f2352c51e3c0/12936_2017_2170_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbc7/5755288/bc3ba5703842/12936_2017_2170_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbc7/5755288/17e8344124db/12936_2017_2170_Fig4_HTML.jpg

相似文献

1
Estimation on local transmission of malaria by serological approach under low transmission setting in Myanmar.缅甸低传播环境下血清学方法估算疟疾本地传播
Malar J. 2018 Jan 5;17(1):6. doi: 10.1186/s12936-017-2170-8.
2
Geographical heterogeneity in prevalence of subclinical malaria infections at sentinel endemic sites of Myanmar.缅甸哨点流行地区亚临床疟疾感染的地理异质性。
Parasit Vectors. 2019 Feb 18;12(1):83. doi: 10.1186/s13071-019-3330-1.
3
Geographical patterns of malaria transmission based on serological markers for falciparum and vivax malaria in Ratanakiri, Cambodia.基于柬埔寨腊塔纳基里省恶性疟和间日疟血清学标志物的疟疾传播地理模式。
Malar J. 2016 Oct 19;15(1):510. doi: 10.1186/s12936-016-1558-1.
4
Common asymptomatic and submicroscopic malaria infections in Western Thailand revealed in longitudinal molecular and serological studies: a challenge to malaria elimination.泰国西部纵向分子和血清学研究揭示的常见无症状和亚显微疟原虫感染：疟疾消除面临的挑战
Malar J. 2016 Jun 22;15:333. doi: 10.1186/s12936-016-1393-4.
5
Malaria epidemiology in central Myanmar: identification of a multi-species asymptomatic reservoir of infection.缅甸中部的疟疾流行病学：发现多物种无症状感染源
Malar J. 2017 Jan 5;16(1):16. doi: 10.1186/s12936-016-1651-5.
6
Asymptomatic and sub-microscopic malaria infection in Kayah State, eastern Myanmar.缅甸东部克耶邦的无症状和亚显微疟原虫感染
Malar J. 2017 Apr 4;16(1):138. doi: 10.1186/s12936-017-1789-9.
7
Multi-epitope chimeric antigen used as a serological marker to estimate Plasmodium falciparum transmission intensity in the border area of China-Myanmar.多表位嵌合抗原用作血清学标志物以评估中缅边境地区恶性疟原虫的传播强度。
Infect Dis Poverty. 2016 Sep 7;5(1):98. doi: 10.1186/s40249-016-0194-x.
8
Characterization of Plasmodium falciparum and Plasmodium vivax recent exposure in an area of significantly decreased transmission intensity in Central Vietnam.中越交界地区疟疾传播强度显著降低背景下恶性疟原虫和间日疟原虫近期感染的特征。
Malar J. 2018 Apr 27;17(1):180. doi: 10.1186/s12936-018-2326-1.
9
Operational Performance of a Plasmodium falciparum Ultrasensitive Rapid Diagnostic Test for Detection of Asymptomatic Infections in Eastern Myanmar.缅甸东部应用一种高灵敏度恶性疟原虫快速诊断检测试剂用于无症状感染检测的操作性能评估。
J Clin Microbiol. 2018 Jul 26;56(8). doi: 10.1128/JCM.00565-18. Print 2018 Aug.
10
Prevalence of Clinical and Subclinical and Malaria in Two Remote Rural Communities on the Myanmar-China Border.中缅边境两个偏远农村社区临床、亚临床疟疾的患病率
Am J Trop Med Hyg. 2017 Nov;97(5):1524-1531. doi: 10.4269/ajtmh.17-0167. Epub 2017 Oct 10.

引用本文的文献

1
Using Serological Markers for the Surveillance of Malaria: A Scoping Review.利用血清学标志物监测疟疾：一项范围综述
Pathogens. 2023 May 31;12(6):791. doi: 10.3390/pathogens12060791.
2
Surveillance of Plasmodium vivax transmission using serological models in the border areas of China-Myanmar.中缅边境地区应用血清学模型监测间日疟原虫传播。
Malar J. 2022 Mar 3;21(1):69. doi: 10.1186/s12936-022-04096-8.
3
Surveillance on the Vivax Malaria in Endemic Areas in the Republic of Korea Based on Molecular and Serological Analyses.

本文引用的文献

1
Molecular surveillance of artemisinin resistance falciparum malaria among migrant goldmine workers in Myanmar.缅甸金矿流动工人中恶性疟原虫青蒿素耐药性的分子监测
Malar J. 2017 Mar 1;16(1):97. doi: 10.1186/s12936-017-1753-8.
2
Molecular Evidence of Drug Resistance in Asymptomatic Malaria Infections, Myanmar, 2015.2015年缅甸无症状疟疾感染中耐药性的分子证据
Emerg Infect Dis. 2017 Mar;23(3):517-520. doi: 10.3201/eid2303.161363.
3
Geographical patterns of malaria transmission based on serological markers for falciparum and vivax malaria in Ratanakiri, Cambodia.
基于分子和血清学分析的韩国地方性流行区间日疟监测。
Korean J Parasitol. 2020 Dec;58(6):609-617. doi: 10.3347/kjp.2020.58.6.609. Epub 2020 Dec 29.
4
School-Based Serosurveys to Assess the Validity of Using Routine Health Facility Data to Target Malaria Interventions in the Central Highlands of Madagascar.基于学校的血清学调查评估利用常规卫生机构数据为马达加斯加中央高地的疟疾干预措施提供目标的有效性。
J Infect Dis. 2021 Mar 29;223(6):995-1004. doi: 10.1093/infdis/jiaa476.
基于柬埔寨腊塔纳基里省恶性疟和间日疟血清学标志物的疟疾传播地理模式。
Malar J. 2016 Oct 19;15(1):510. doi: 10.1186/s12936-016-1558-1.
4
Functional Antibodies and Protection against Blood-stage Malaria.功能性抗体与抗血液期疟疾保护作用
Trends Parasitol. 2016 Nov;32(11):887-898. doi: 10.1016/j.pt.2016.07.003. Epub 2016 Aug 18.
5
Effective High-Throughput Blood Pooling Strategy before DNA Extraction for Detection of Malaria in Low-Transmission Settings.在低传播环境中进行DNA提取前用于疟疾检测的有效高通量血液汇集策略。
Korean J Parasitol. 2016 Jun;54(3):253-9. doi: 10.3347/kjp.2016.54.3.253. Epub 2016 Jun 30.
6
Referencing cross-reactivity of detection antibodies for protein array experiments.蛋白质阵列实验检测抗体的交叉反应性引用。
F1000Res. 2016 Jan 18;5:73. doi: 10.12688/f1000research.7668.2. eCollection 2016.
7
Common asymptomatic and submicroscopic malaria infections in Western Thailand revealed in longitudinal molecular and serological studies: a challenge to malaria elimination.泰国西部纵向分子和血清学研究揭示的常见无症状和亚显微疟原虫感染：疟疾消除面临的挑战
Malar J. 2016 Jun 22;15:333. doi: 10.1186/s12936-016-1393-4.
8
Immunogenetic markers associated with a naturally acquired humoral immune response against an N-terminal antigen of Plasmodium vivax merozoite surface protein 1 (PvMSP-1).与针对间日疟原虫裂殖子表面蛋白1（PvMSP-1）N端抗原的自然获得性体液免疫反应相关的免疫遗传标记。
Malar J. 2016 Jun 3;15:306. doi: 10.1186/s12936-016-1350-2.
9
Sparse serological evidence of Plasmodium vivax transmission in the Ouest and Sud-Est departments of Haiti.海地西部和东南部地区间日疟原虫传播的血清学证据稀少。
Acta Trop. 2016 Oct;162:27-34. doi: 10.1016/j.actatropica.2016.05.011. Epub 2016 May 24.
10
Broadly neutralizing epitopes in the Plasmodium vivax vaccine candidate Duffy Binding Protein.间日疟原虫疫苗候选物达菲结合蛋白中的广泛中和表位
Proc Natl Acad Sci U S A. 2016 May 31;113(22):6277-82. doi: 10.1073/pnas.1600488113. Epub 2016 May 18.