Huang Yu-Lin, Wang Huan-Jing, Chen Fu-Mei, Zhao Xiu-Ling, Fu Qiang, Zhang Peng-Fei, Pu Li-Ping, Huang Feng-Ling, Lu Yang-Qing, Zhang Ming
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning 530004, Guangxi, PR China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, PR China.
Reproductive Center, General Hospital of People's Liberation Army Air Force, Beijing 100142, PR China.
Theriogenology. 2018 Apr 1;110:1-7. doi: 10.1016/j.theriogenology.2017.12.040. Epub 2017 Dec 30.
It has been reported that BCL2L10 is abundantly and specifically expressed in adult human and mouse oocytes and played a very important role in oocytes maturation and early embryonic development. This study is to investigate the expression pattern of BCL2L10 in buffalo ovaries and its effect on the in vitro maturation of buffalo oocytes, so as to dissect mechanism of oocytes maturation and provide theoretical guidance for improvement of the in vitro maturation of buffalo oocytes. The results showed that BCL2L10 gene was enriched in ovary and the expression of BCL2L10 was oocyte specific and up-regulated during oocyte maturation. BCL2L10 protein and mRNA were detectable in buffalo early embryos, upregulated at 2-cell to 8-cell stages and down-regulated in the later stages. Knockdown of BCL2L10 by RNA interference resulted in a significant decrease in the maturation rate (33.5%) and cleavage rate (37.52%) of buffalo oocytes coupled with up-regulation of apoptosis-related gene Caspase-9. We concluded that BCL2L10 is a candidate associated with buffalo oocyte maturation.
据报道，BCL2L10在成年人类和小鼠卵母细胞中大量且特异性表达，在卵母细胞成熟和早期胚胎发育中发挥着非常重要的作用。本研究旨在探讨BCL2L10在水牛卵巢中的表达模式及其对水牛卵母细胞体外成熟的影响，以剖析卵母细胞成熟机制，并为提高水牛卵母细胞体外成熟率提供理论指导。结果表明，BCL2L10基因在卵巢中富集，其表达具有卵母细胞特异性，且在卵母细胞成熟过程中上调。在水牛早期胚胎中可检测到BCL2L10蛋白和mRNA，在2细胞至8细胞阶段上调，后期下调。通过RNA干扰敲低BCL2L10导致水牛卵母细胞成熟率（33.5%）和卵裂率（37.52%）显著降低，同时凋亡相关基因Caspase-9上调。我们得出结论，BCL2L10是与水牛卵母细胞成熟相关的一个候选基因。
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