从一名散发性帕金森病患者中生成无整合诱导多能干细胞系（CSC-43）。
Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease.
作者信息
Marote Ana, Pomeshchik Yuriy, Goldwurm Stefano, Collin Anna, Lamas Nuno J, Pinto Luísa, Salgado António J, Roybon Laurent
机构信息
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Stem Cell Laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, Lund, Sweden; Strategic Research Area MultiPark, Lund University, Lund, Sweden; Lund Stem Cell Center, Lund University, Lund, Sweden.
出版信息
Stem Cell Res. 2018 Mar;27:82-85. doi: 10.1016/j.scr.2018.01.007. Epub 2018 Jan 4.
An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of common pluripotency markers, in vitro differentiation into three germ layers and normal karyotype. This iPSC line can be used to study the mechanisms underlying the development of PD.
从一名36岁的散发性帕金森病（PD）患者身上产生了诱导多能干细胞（iPSC）系。使用非整合型仙台病毒技术对皮肤成纤维细胞进行重编程，以导入OCT3/4、SOX2、c-MYC和KLF4因子。所产生的细胞系（CSC-43）表现出常见多能性标志物的表达、在体外分化为三个胚层以及正常的核型。该iPSC系可用于研究帕金森病发生发展的潜在机制。
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