一种检测烟曲霉唑类耐药菌株中cyp51A启动子串联重复序列的简单方法。

A simple method to detect the tandem repeat of the cyp51A promoter in azole-resistant strains of Aspergillus fumigatus.

作者信息

Toyotome Takahito, Hagiwara Daisuke, Watanabe Akira, Kamei Katsuhiko

机构信息

Obihiro University of Agriculture and Veterinary Medicine, 11, Nishi 2, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.

Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba City, Chiba 260-8673, Japan.

出版信息

Med Mycol. 2018 Nov 1;56(8):1042-1044. doi: 10.1093/mmy/myx149.

DOI:10.1093/mmy/myx149

PMID:29340615

Abstract

We designed primers and cycling probes to detect the tandem repeat (TR) of cyp51A promoter in Aspergillus fumigatus. A control-probe was designed to anneal to the outside of the TR region, whereas a TR-probe was designed to anneal to the inside of the TR region. For amplification and probe-hydrolysis detection, the CycleavePCR system was used. Although the difference between Ct values of the wild-type genome for the control-probe and the TR-probe was around -0.1, the difference between Ct values of TR-harboring strains was around 0.7. These data indicate that this is a simple method to detect TR in azole-resistant A. fumigatus.

摘要

我们设计了引物和循环探针来检测烟曲霉中cyp51A启动子的串联重复序列（TR）。设计了一个对照探针，使其退火到TR区域之外，而设计了一个TR探针，使其退火到TR区域之内。为了进行扩增和探针水解检测，使用了CycleavePCR系统。虽然对照探针和TR探针的野生型基因组Ct值之差约为-0.1，但携带TR的菌株的Ct值之差约为0.7。这些数据表明，这是一种检测耐唑类烟曲霉中TR的简单方法。