Raza Asad, Ansari Tariq Mahmood
Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan.
Pak J Pharm Sci. 2018 Jan;31(1):153-157.
In this study we have reported a new, fast and extraction free spectrophotometric procedure for the assessment of caroverine in pharmaceutical raw and tablet dosage forms. In the reported visible spectrophotometric procedure tungstate in Folin-Ciocalteu phenol reagent is reduced in alkaline medium and produces the blue colored chromogen that shows Λ at 740nm with the calibration range of 2-28μg/ml. The LOD and LOQ values are 1.15 and 3.81μg/ml respectively. The newly developed analytical procedure is used to determine caroverine in raw material of and commercial tablets dosage forms. The spectrophotometric method represented in this study is simple, rapid and extraction free. It may easily be utilized for the determination of caroverine in pharmaceutical laboratories for quality control and stability studies purpose.
在本研究中，我们报道了一种用于评估原料药和片剂剂型中卡维林的新型、快速且无需萃取的分光光度法。在所报道的可见分光光度法中，福林-西奥尔特酚试剂中的钨酸盐在碱性介质中被还原，生成在740nm处有吸收峰的蓝色色原，校准范围为2-28μg/ml。检测限和定量限分别为1.15和3.81μg/ml。新开发的分析方法用于测定原料药和市售片剂剂型中的卡维林。本研究中所呈现的分光光度法简单、快速且无需萃取。它可轻松用于制药实验室中卡维林的测定，以进行质量控制和稳定性研究。
