Cathepsin B & D and the Survival of Early Embryos in Red Spotted Grouper, .

Survival of embryos largely depends on yolk processing during early development. Proteolytic enzymes, cathepsin B & D ( & ) are known to have some important roles in yolk processing of various fish species. Mature female red spotted groupers were injected with human chorionic gonadotropin (HCG) to induce ovulation. The fertilized eggs and embryos were sampled at 0, 4 and 24 HPF (hours post fertilization). Survivals of each groups of embryos were checked at 24 and 48 HPH (hours post hatching). Transcripts of & showed the highest level at 0 HPF and relatively high at 4 HPF, but greatly decreased at 24 HPF. In bad egg quality group (BE, embryos survived until 24 HPH), transcript level of at 4 HPF were significantly lower than the transcript level at the same stage in good egg quality group (GE, embryos survived until 48 HPH) while no significant change of transcript level was observed at 0 or 24 HPF between BE and GE. Transcript level of was decreased at 24 HPF, but the difference was not as strong as the case of transcript. These results suggest that maternal transcript rather than transcript is likely to be involved in egg quality resulting in the difference of survival rate of embryos at early developmental period in this species.