Guo Xinrong, Zhang Jingjing, Ma Xiaojun, Ma Xiaowei
College of Acupuncture-Moxibustion and Tuina, Shaanxi University of CM, Xianyang 712046, China; Research Center of Acupuncture-Moxibustion and Tuina, Tanjin University of TCM, Tianjin 300193, China.
College of Acupuncture-Moxibustion and Tuina, Shaanxi University of CM, Xianyang 712046, China.
Zhongguo Zhen Jiu. 2018 Jan 12;38(1):65-9. doi: 10.13703/j.0255-2930.2018.01.016.
To explore the correlation between efficacy of electroacupuncture (EA) on mammary gland hyperplasia (MGH) and the regulatory pathway of intercostal nerve.
Fifty female SD rats were randomly divided into a blank group (group A, 12 rats), a model group (group B, 12 rats), an EA group (group C, 13 rats) and an intercostal nerve transection group (group D, 13 rats). The rats in the group B, group C and group D were prepared into MGH model; after model was successfully prepared, the 7th intercostal nerve was cut off in the group D. EA was applied at back acupoints including bilateral "Tianzong" (SI 11), "Ganshu" (BL 18) and "Shenshu" (BL 23) as well as chest acupoints including bilateral "Wuyi" (ST 15), "Hegu" (LI 4) and "Danzhong" (CV 17) in the group C and D. The two groups of acupoints were selected alternately. EA was given for 20 min, once a day; 5-day treatment was taken as one course; there was an interval of 2 days between course; totally 20 treatments were given. After treatment, the height and diameter of papilla were observed; the contents of serum estradiol (E) and progestin (P), the expression of estrogen receptor α (ERα) and progestrone receptor (PR) in mammary gland were measured.
(1) The height and diameter of papilla: after treatment, the height and diameter of papilla in the group C were significantly smaller than those in the group B (both <0.05); the height and diameter of left-side papilla in the group D were significantly bigger than those in the group C (both <0.05). (2) Serum E and P: after treatment, compared with the group B, the contents of E and E/P were reduced and the content of P was increased in the group C and group D (all <0.05). Compared with the group C, the contents of E and E/P were increased and the content of P was reduced in the group D (all <0.05). (3) ERα and PR in mammary gland: compared with the group B, the content of ERαwas decreased and the content of PR was increased in the group C (both <0.05). Compared with the group C, the content of ERαwas increased and the content of PR was decreased in the group D ((both <0.05).
The efficacy mechanism of EA for MGH is likely to be related with the pathway of intercostal nerve; the mechanism may be acupuncture regulating the contents of serum E and P as well as contents of ERα and PR in mammary gland.
探讨电针（EA）治疗乳腺增生（MGH）的疗效与肋间神经调节通路之间的相关性。
将50只雌性SD大鼠随机分为空白组（A组，12只）、模型组（B组，12只）、电针组（C组，13只）和肋间神经切断组（D组，13只）。B组、C组和D组大鼠制备MGH模型；模型成功制备后，D组切断第7肋间神经。C组和D组在背部穴位包括双侧“天宗”（SI 11）、“肝俞”（BL 18）和“肾俞”（BL 23）以及胸部穴位包括双侧“屋翳”（ST 15）、“合谷”（LI 4）和“膻中”（CV 17）进行电针。两组穴位交替选取。电针20分钟，每日1次；5天治疗为1个疗程；疗程间间隔2天；共给予20次治疗。治疗后，观察乳头高度和直径；检测血清雌二醇（E）和孕激素（P）含量、乳腺组织中雌激素受体α（ERα）和孕激素受体（PR）的表达。
（1）乳头高度和直径：治疗后，C组乳头高度和直径均显著小于B组（均P<0.05）；D组左侧乳头高度和直径均显著大于C组（均P<0.05）。（2）血清E和P：治疗后，与B组比较，C组和D组E及E/P含量降低，P含量升高（均P<0.05）。与C组比较，D组E及E/P含量升高，P含量降低（均P<0.05）。（3）乳腺组织中ERα和PR：与B组比较，C组ERα含量降低，PR含量升高（均P<0.05）。与C组比较，D组ERα含量升高，PR含量降低（均P<0.05）。
电针治疗MGH的疗效机制可能与肋间神经通路有关；其机制可能是针刺调节血清E和P含量以及乳腺组织中ERα和PR含量。
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