Dusek Robert J, Justice-Allen Anne, Bodenstein Barbara, Knowles Susan, Grear Daniel A, Adams Laura, Levy Craig, Yaglom Hayley D, Shearn-Bochsler Valerie I, Ciembor Paula G, Gregory Christopher R, Pesti Denise, Ritchie Branson W
1 US Geological Survey, National Wildlife Health Center, 6006 Schroeder Road, Madison, Wisconsin 53711, USA.
2 Arizona Game and Fish Department, 5000 W Carefree Highway, Phoenix, Arizona 85086, USA.
J Wildl Dis. 2018 Apr;54(2):248-260. doi: 10.7589/2017-06-145. Epub 2018 Jan 25.
In 2013, a mortality event of nonnative, feral Rosy-faced Lovebirds ( Agapornis roseicollis) in residential backyards in Maricopa County, Arizona, US was attributed to infection with Chlamydia psittaci. In June 2014, additional mortality occurred in the same region. Accordingly, in August 2014 we sampled live lovebirds and sympatric bird species visiting backyard bird feeders to determine the prevalence of DNA and the seroprevalence of antibodies to C. psittaci using real-time PCR-based testing and elementary body agglutination, respectively. Chlamydia psittaci DNA was present in conjunctival-choanal or cloacal swabs in 93% (43/46) of lovebirds and 10% (14/142) of sympatric birds. Antibodies to C. psittaci were detected in 76% (31/41) of lovebirds and 7% (7/102) of sympatric birds. Among the sympatric birds, Rock Doves ( Columba livia) had the highest prevalence of C. psittaci DNA (75%; 6/8) and seroprevalence (25%; 2/8). Psittacine circovirus 1 DNA was also identified, using real-time PCR-based testing, from the same swab samples in 69% (11/16) of species sampled, with a prevalence of 80% (37/46) in lovebirds and 27% (38/142) in sympatric species. The presence of either Rosy-faced Lovebirds or Rock Doves at residential bird feeders may be cause for concern for epizootic and zoonotic transmission of C. psittaci in this region.
2013年，美国亚利桑那州马里科帕县居民区后院出现了非本地野生玫瑰脸情侣鹦鹉（Agapornis roseicollis）死亡事件，原因是感染了鹦鹉热衣原体。2014年6月，同一地区又出现了更多死亡病例。因此，2014年8月，我们对在后院鸟类喂食器处觅食的活情侣鹦鹉和同域鸟类物种进行了采样，分别使用基于实时PCR的检测方法和原体凝集试验来确定鹦鹉热衣原体DNA的流行率和抗体血清阳性率。在93%（43/46）的情侣鹦鹉和10%（14/142）的同域鸟类的结膜-鼻后孔或泄殖腔拭子中检测到鹦鹉热衣原体DNA。在76%（31/41）的情侣鹦鹉和7%（7/102）的同域鸟类中检测到鹦鹉热衣原体抗体。在同域鸟类中，原鸽（Columba livia）的鹦鹉热衣原体DNA流行率最高（75%；6/8），血清阳性率也最高（25%；2/8）。使用基于实时PCR的检测方法，还从69%（11/16）的采样物种的相同拭子样本中鉴定出了鹦鹉圆环病毒1 DNA，其中情侣鹦鹉的流行率为80%（37/46），同域物种的流行率为27%（38/142）。居民区鸟类喂食器处出现玫瑰脸情侣鹦鹉或原鸽可能会引发该地区鹦鹉热衣原体的动物流行病和人畜共患病传播问题。
