实验性自身免疫性重症肌无力中针对乙酰胆碱受体的抑制性细胞的诱导

Induction of suppressor cells specific for AChR in experimental autoimmune myasthenia gravis.

作者信息

McIntosh K R, Drachman D B

出版信息

Science. 1986 Apr 18;232(4748):401-3. doi: 10.1126/science.2938256.

Abstract

Suppressor cells specific for acetylcholine receptor (AChR) were induced in a population of lymphocytes previously sensitized to AChR, obtained from rats with experimental autoimmune myasthenia gravis (EAMG). The lymphocytes were cultured with the immunosuppressive drug cyclosporin A plus purified AChR for 7 days. These cells, when mixed with lymphocytes from rats with EAMG in vitro, strongly suppressed the antibody response to AChR. They did not inhibit antibody responses to an unrelated antigen, an indication that suppression was specific for AChR. This approach should be a useful way to induce specific suppressor cells from sensitized populations of lymphocytes and may be applicable in the treatment of autoimmune diseases such as myasthenia gravis.

摘要

在患有实验性自身免疫性重症肌无力（EAMG）的大鼠中获取预先对乙酰胆碱受体（AChR）致敏的淋巴细胞群体，诱导出了对AChR具有特异性的抑制细胞。将这些淋巴细胞与免疫抑制药物环孢素A及纯化的AChR一起培养7天。这些细胞在体外与来自患有EAMG的大鼠的淋巴细胞混合时，强烈抑制了对AChR的抗体反应。它们并未抑制对无关抗原的抗体反应，这表明这种抑制作用对AChR具有特异性。这种方法应该是从致敏淋巴细胞群体中诱导特异性抑制细胞的一种有用方式，并且可能适用于治疗重症肌无力等自身免疫性疾病。