TALP和柠檬酸三羟甲基氨基甲烷培养基对山羊精子获能及后续胚胎生产的研究

Study of TALP and TRIS citrate medium on caprine sperm capacitation and subsequent embryo production.

作者信息

Agarwal S, Kharche S D, Bhatiya A K

机构信息

Ph.D. Scholar in Biotechnology, Department of Biotechnology, Institute of Applied Science and Humanities, GLA University, Mathura, Uttar Pradesh, India.

Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India.

出版信息

Iran J Vet Res. 2017 Fall;18(4):275-278.

PMID:29387101

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5767635/

Abstract

The aim of the present investigation was to compare Tyrode's albumin lactate pyruvate (TALP) medium and TRIS citrate medium for capacitation of caprine sperm. In experiment 1 capacitation was assessed by chlortetracycline assay and in experiment 2 with fertilization and embryo development. In experiment 2, cumulus oocyte complexes (COCs) recovered by slicing the caprine ovaries were matured in maturation medium for 27 h in humidified atmosphere at 38.5°C with 5% CO. After 27 h of culture a total of 2480 matured oocytes were selected and randomly divided into two groups. Group 1 (n=1124) matured oocytes were fertilized by the spermatozoa capacitated in TALP medium and in group 2 (n=1356) matured oocytes were fertilized by the spermatozoa capacitated in TRIS citrate medium. The results of experiment 1 indicated a comparatively more number of sperms with Chlortetracycline (CTC) Pattern B in TRIS citrate than TALP medium (55.32 ± 0.91% vs 47.96 ± 0.20%). In experiment 2, the cleavage rate and blastocyst production were higher following capacitation of spermatozoa in TRIS citrate than TALP medium. In conclusion, TRIS citrate can be used as an alternative and effective media for sperm capacitation to get higher cleavage rate and blastocyst production in goat.

摘要

本研究的目的是比较台氏白蛋白乳酸丙酮酸（TALP）培养基和柠檬酸三羟甲基氨基甲烷（TRIS）培养基对山羊精子获能的影响。实验1通过金霉素检测评估精子获能情况，实验2则观察受精和胚胎发育情况。在实验2中，通过切割山羊卵巢收集卵丘卵母细胞复合体（COCs），将其在成熟培养基中于38.5°C、5%二氧化碳的湿润环境中培养27小时。培养27小时后，共挑选出2480个成熟卵母细胞并随机分为两组。第1组（n = 1124）的成熟卵母细胞由在TALP培养基中获能的精子受精，第2组（n = 1356）的成熟卵母细胞由在柠檬酸三羟甲基氨基甲烷（TRIS）培养基中获能的精子受精。实验1的结果表明，与TALP培养基相比，柠檬酸三羟甲基氨基甲烷（TRIS）培养基中呈现金霉素（CTC）模式B的精子数量相对较多（55.32 ± 0.91%对47.96 ± 0.20%）。在实验2中，与TALP培养基相比，精子在柠檬酸三羟甲基氨基甲烷（TRIS）培养基中获能后，卵裂率和囊胚形成率更高。总之，柠檬酸三羟甲基氨基甲烷（TRIS）培养基可作为一种替代且有效的精子获能培养基，以提高山羊的卵裂率和囊胚形成率。