使用逆转录病毒载体在小鼠原代B细胞和B细胞系中表达外源基因。

Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors.

作者信息

Yang Zhiyong, Allen Christopher D C

机构信息

Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, 94143, USA.

Sandler Asthma Basic Research Center, University of California, San Francisco, San Francisco, CA, 94143, USA.

出版信息

Methods Mol Biol. 2018;1707:39-49. doi: 10.1007/978-1-4939-7474-0_3.

DOI:10.1007/978-1-4939-7474-0_3

PMID:29388098

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6675621/

Abstract

B cells, after activation, can undergo class-switch recombination and somatic hypermutation of their immunoglobulin genes, and can differentiate into memory cells and plasma cells. Expressing genes in altered versions in primary B cells and B cell lines is an important approach to understanding how B cell receptor signaling leads to B cell activation and differentiation. Recombinant retrovirus-based transduction is the most efficient method to deliver exogenous genes for expression in B cells. In this chapter, we describe streamlined protocols for using recombinant retroviral vectors to transduce both murine primary B cells and B cell lines.

摘要

B细胞活化后，可发生免疫球蛋白基因的类别转换重排和体细胞高频突变，并可分化为记忆细胞和浆细胞。在原代B细胞和B细胞系中以改变的形式表达基因，是理解B细胞受体信号如何导致B细胞活化和分化的重要方法。基于重组逆转录病毒的转导是在B细胞中表达外源基因的最有效方法。在本章中，我们描述了使用重组逆转录病毒载体转导小鼠原代B细胞和B细胞系的简化方案。