Gong Yi, Li Yunkai, Chen Xinjun, Chen Ling
College of Marine Sciences, Shanghai Ocean University, 999 Huchenghuan Rd, Shanghai, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 106 Nanjing Rd, Qingdao, China.
Rapid Commun Mass Spectrom. 2018 Apr 15;32(7):583-589. doi: 10.1002/rcm.8071.
Squid is an important seafood resource for Asian and European countries. With the continuous development of processed squid products, an effective traceability system has become increasingly prominent. Here, we attempt to trace the fishery products of the main target species, jumbo squid (Dosidicus gigas), by using biochemical tracers.
Carbon and nitrogen isotope ratios (δ C and δ N values) and fatty acid profiles were identified in squid from three harvest locations in the eastern Pacific Ocean by isotope ratio mass spectrometry and gas chromatography/mass spectrometry, respectively. Comparative analysis was used to evaluate the geographic variations in tracers and to identify the suitable discriminatory variables among origins.
Significant spatial variations were found in isotopic values and fatty acid profiles in squid muscle tissues, possibly because of different food availability and/or oceanographic conditions that each group experiences at a given location. The stepwise discriminant analysis indicated that δ N, C16:1n7, C17:1n7, C18:2n6, C20:1 and C20:4n6 were effective variables at differentiating origin.
Combined use of stable isotope ratios and fatty acid analyses could trace geographic origins of jumbo squid. This study provides an alternative approach for improving authenticity evaluation of commercial squid products.
鱿鱼是亚洲和欧洲国家重要的海鲜资源。随着鱿鱼加工产品的不断发展，有效的可追溯系统变得越来越重要。在此，我们尝试通过使用生化示踪剂来追踪主要目标物种——巨型鱿鱼（茎柔鱼）的渔业产品。
分别通过同位素比率质谱法和气相色谱/质谱法，对东太平洋三个捕捞地点的鱿鱼的碳和氮同位素比率（δC和δN值）以及脂肪酸谱进行了鉴定。采用比较分析来评估示踪剂的地理差异，并确定不同产地之间合适的鉴别变量。
在鱿鱼肌肉组织中，同位素值和脂肪酸谱存在显著的空间差异，这可能是由于每组在特定位置所经历的食物可获得性和/或海洋条件不同所致。逐步判别分析表明，δN、C16:1n7、C17:1n7、C18:2n6、C20:1和C20:4n6是区分产地的有效变量。
稳定同位素比率和脂肪酸分析的联合使用可以追踪巨型鱿鱼的地理来源。本研究为提高商业鱿鱼产品的真实性评估提供了一种替代方法。
