Scherer Stephanie, Göbel Thomas W
Institute for Animal Physiology, Department of Veterinary Sciences, University of Munich, Veterinärstrasse 13, 80539 Munich, Germany.
Institute for Animal Physiology, Department of Veterinary Sciences, University of Munich, Veterinärstrasse 13, 80539 Munich, Germany.
Dev Comp Immunol. 2018 May;82:128-138. doi: 10.1016/j.dci.2018.01.014. Epub 2018 Feb 3.
The Tumour Necrosis Factor superfamilies of receptors and ligands play a crucial role in the regulation of effective immune responses against pathogens and malignant cells. In chickens, only few members have been identified. Here, we characterise the chicken homologues for mammalian costimulatory molecules OX40 and OX40L, which are involved in sustaining T cell responses. Both genes were identified by virtue of their genomic localisation close to highly conserved genes and their structural relationship to their mammalian homologues. Following cloning and expression of soluble and cell-associated chicken OX40 and OX40L, we confirmed their mutual interaction via ELISA and flow cytometric analyses. In addition, we showed the application of soluble OX40-Fc in staining of chicken cells. Whereas non-activated cells did not express OX40L, activation by IL-2 and IL-12 resulted in upregulation of OX40L on αβ and γδ T cell populations. Our results demonstrate the existence of the costimulatory OX40-OX40L system in the chicken and provide the basis for further investigations of chicken T cell responses.
肿瘤坏死因子受体和配体超家族在调节针对病原体和恶性细胞的有效免疫反应中起着关键作用。在鸡中，仅鉴定出少数成员。在此，我们对哺乳动物共刺激分子OX40和OX40L的鸡同源物进行了表征，它们参与维持T细胞反应。这两个基因是根据它们在基因组中靠近高度保守基因的定位以及它们与哺乳动物同源物的结构关系而鉴定出来的。在克隆并表达了可溶性和细胞相关的鸡OX40和OX40L之后，我们通过ELISA和流式细胞术分析证实了它们之间的相互作用。此外，我们展示了可溶性OX40-Fc在鸡细胞染色中的应用。未活化的细胞不表达OX40L，而IL-2和IL-12激活导致αβ和γδ T细胞群体上OX40L上调。我们的结果证明了鸡中存在共刺激OX40-OX40L系统，并为进一步研究鸡T细胞反应提供了基础。
