Department of Molecular Parasitology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon, Korea.
Department of Environmental Medical Biology, Yonsei University College of Medicine, Seoul, Korea.
Proteomics. 2018 Apr;18(7):e1700341. doi: 10.1002/pmic.201700341. Epub 2018 Mar 7.
The obligatory intracellular protozoan parasite Entamoeba histolytica causes amebic dysentery and liver abscess. E. histolytica adheres to the host tissues in a contact-dependent manner. E. histolytica excretory-secretory products (ESP) might play critical roles during invasion. We comparatively analyzed the secretome profile of E. histolytica pathogenic HM-1:IMSS and non-pathogenic Rahman strains. The two ESP revealed similar but distinct spotting patterns. In both ESP, alcohol dehydrogenase, enolase 1, and transketolase, which control classical carbohydrate metabolism and other moonlighting effects, constituted the most abundant fractions. We recognized differently secreted molecules. Secretion of cytoskeletal organization proteins (actin, actin binding protein, and EHI_068510), protein remodeling amino peptidase, and multifunctional elongation factor 1-α were increased in Rahman. Conversely, carbohydrate metabolizing enolase 1, alcohol dehydrogenase, transketolase, calponin, phosphoglucose mutase, malic enzyme and EHI_156420, xenobiotic scavenging superoxide dismutase and EHI_140740, and pyruvate:ferredoxin oxidoreductase and coronin (carbohydrate metabolism/detoxification) showed reduced secretion. Transcription levels of some genes involved in these processes also decreased. Changes of secretory behavior, especially decreased secretion of multifunctional carbohydrate metabolizing enzymes and detoxifying proteins that importantly participated in amoeba pathogenesis might reflect avirulent nature of Rahman strain in the host.
内共生的原生动物寄生虫溶组织内阿米巴会引起阿米巴痢疾和肝脓肿。溶组织内阿米巴以接触依赖的方式黏附在宿主组织上。溶组织内阿米巴分泌的外泌体（ESP）可能在入侵过程中发挥关键作用。我们比较分析了致病性 HM-1:IMSS 和非致病性 Rahman 株的溶组织内阿米巴 secretome 图谱。两种 ESP 显示出相似但不同的斑点模式。在两种 ESP 中，控制经典碳水化合物代谢和其他 moonlighting 效应的醇脱氢酶、烯醇酶 1 和转酮醇酶构成了最丰富的部分。我们识别出了不同分泌的分子。细胞骨架组织蛋白（肌动蛋白、肌动蛋白结合蛋白和 EHI_068510）、蛋白重塑氨肽酶和多功能延伸因子 1-α的分泌在 Rahman 中增加。相反，碳水化合物代谢烯醇酶 1、醇脱氢酶、转酮醇酶、钙调蛋白、磷酸葡萄糖变位酶、苹果酸酶和 EHI_156420、外源性化合物清除超氧化物歧化酶和 EHI_140740 以及丙酮酸：铁氧还蛋白氧化还原酶和冠蛋白（碳水化合物代谢/解毒）的分泌减少。这些过程中一些参与的基因的转录水平也降低了。分泌行为的变化，特别是多功能碳水化合物代谢酶和解毒蛋白的分泌减少，这些蛋白在阿米巴病发病机制中起着重要作用，可能反映了 Rahman 株在宿主中的无毒性。
