Buch Pranav R, Desai Isha, Balakrishnan Suresh
Department of Zoology, Faculty of Science, The M. S. University of Baroda, Vadodara, 390002, Gujarat, India.
N.V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, Anand, 388120, Gujarat, India.
Prostaglandins Other Lipid Mediat. 2018 Mar;135:11-15. doi: 10.1016/j.prostaglandins.2018.01.002. Epub 2018 Feb 4.
Cyclooxygenase-2 (COX-2) is an important mediator of the immune response. It is found upregulated after pathogen invasion or tissue injury and also in many cancers. Of the lesser known functions of this enzyme is its role in effecting epimorphic regeneration. We have previously shown that COX-2 activity is essential for proper regeneration of tail in lizard Hemidactylus flaviviridis; however, the pattern of its activity and expression during the early stages of regeneration was unknown. The present work provides the first report of the trend in COX-2 activity and expression during the wound healing in epimorphic regeneration. It was found in H. flaviviridis that COX-2 gene was induced on the first day after amputation of the tail and expression and activity remained high through the course of wound healing. Further it was revealed that the COX-2 signal was mediated through the PKA/cAMP pathway via binding with the prostaglandin E receptor 2 (EP2). In order to delineate the mechanism of epimorphic regeneration, we must understand the regulation of the major regulatory molecules therein. Therefore, the current study on the role of COX-2 during the regenerative wound healing is of paramount significance. Optimistically, such a mechanistic insight will help us achieve large scale tissue regeneration in humans in the future.
环氧化酶-2（COX-2）是免疫反应的重要介质。在病原体入侵或组织损伤后以及在许多癌症中均可发现其表达上调。该酶鲜为人知的功能之一是其在影响形态再生中的作用。我们之前已经表明，COX-2活性对于绿鬣蜥尾巴的正常再生至关重要；然而，在再生早期其活性和表达模式尚不清楚。目前的工作首次报道了在形态再生伤口愈合过程中COX-2活性和表达的趋势。在绿鬣蜥中发现，尾巴截肢后第一天COX-2基因被诱导，并且在伤口愈合过程中表达和活性一直保持较高水平。进一步研究表明，COX-2信号是通过与前列腺素E受体2（EP2）结合，经由PKA/cAMP途径介导的。为了阐明形态再生的机制，我们必须了解其中主要调节分子的调控。因此，目前关于COX-2在再生性伤口愈合中作用的研究具有至关重要的意义。乐观地讲，这样的机制性见解将有助于我们未来在人类中实现大规模组织再生。
