Lodin Z, Faltin J, Lisa V, Hartman J, Mares V
Physiol Bohemoslov. 1985;34 Suppl:101-4.
Single stages of histiotypic formation from dissociated embryonic brain cells are described. The first stage, i.e. primary adhesion, is a phenomenon depending mainly on physicochemical features of the adhesive system. The composition of the membrane glycoprotein coat was studied during membrane regeneration and formation of cellular contacts. At the beginning single terminal sugar components and negative sialic acid residues are distributed non-homogeneously over the membrane according to the ligation of lectins. During the formation of cellular contacts this non-homogeneity progressively disappears, the thickness of the layer is reduced, however, the density of single markers increases. The highest increase of both density and layer thickness during the phase of membranes reparation occurs when the negative surface groups are labelled with cationized ferritin. The sorting out process was studied in mixed cultures as a stage of final specific recognition. It is assumed that the reaggregation of cells is a multistep process depending on maturation of the glycoprotein coat, characterized by multiple cellular adhesion and deadhesion, completed by specific recognition and fixation of recognized cells.
描述了从解离的胚胎脑细胞形成组织型的单个阶段。第一阶段，即初级黏附，是一种主要取决于黏附系统物理化学特征的现象。在膜再生和细胞接触形成过程中研究了膜糖蛋白衣的组成。起初，根据凝集素的连接，单个末端糖成分和负性唾液酸残基在膜上分布不均匀。在细胞接触形成过程中，这种不均匀性逐渐消失，层的厚度减小，然而，单个标记物的密度增加。当用阳离子铁蛋白标记负性表面基团时，在膜修复阶段密度和层厚度的增加最为显著。在混合培养物中研究了分选过程，将其作为最终特异性识别的一个阶段。据推测，细胞的重新聚集是一个多步骤过程，取决于糖蛋白衣的成熟，其特征是多次细胞黏附和脱黏附，通过已识别细胞的特异性识别和固定而完成。
