Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Biometals. 2018 Apr;31(2):233-242. doi: 10.1007/s10534-018-0079-5. Epub 2018 Feb 10.
A variety of biological activities, such as anti-microbial and anti-tumor properties was reported for 1,10-phenanthroline and its copper complexes. In this study, the anti-proliferative activity of a novel [Cu(L)(phen)] complex was investigated on MCF-7 breast cancer cells using MTT assay. Since chemotherapy is lake of ability to distinguish between normal cells from cancerous cells, therefore we also investigated the effect of [Cu(L)(phen)] complex on normal L929 cells. The results showed that following 24 and 48 h exposure of cells with [Cu(L)(phen)] complex, the IC50 values for MCF-7 were significantly lower than that recorded for L929 and normal cells were less sensitive than cancerous cells to the complex. Additionally, the [Cu(L)(phen)] complex displayed a time- and concentration-dependent cytotoxic response, with MCF-7 and L929 cells. Also flow cytometry findings suggest that [Cu(L)(phen)] complex is capable of decreasing cancer cell viability through apoptosis and did not efficiently activate the necrosis process.
1,10-菲啰啉及其铜配合物具有多种生物活性，如抗微生物和抗肿瘤特性。在这项研究中，采用 MTT 法研究了新型[Cu(L)(phen)]配合物对 MCF-7 乳腺癌细胞的增殖抑制活性。由于化疗缺乏区分正常细胞和癌细胞的能力，因此我们还研究了[Cu(L)(phen)]配合物对正常 L929 细胞的影响。结果表明，细胞暴露于[Cu(L)(phen)]配合物 24 和 48 h 后，MCF-7 的 IC50 值明显低于 L929 记录的值，正常细胞对该配合物的敏感性低于癌细胞。此外，[Cu(L)(phen)]配合物对 MCF-7 和 L929 细胞表现出时间和浓度依赖性的细胞毒性反应。流式细胞术的研究结果表明，[Cu(L)(phen)]配合物能够通过凋亡降低癌细胞的活力，而不能有效地激活坏死过程。
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