Song Myeong-Sub, Sekhon Simranjeet Singh, Shin Woo-Ri, Rhee Sung-Keun, Ko Jung Ho, Kim Sang Yong, Min Jiho, Ahn Ji-Young, Kim Yang-Hoon
School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju 28644, South Korea.
College of Veterinary Medicine, Western University of Health Sciences, 309 E Second Street, Pomona CA 91766, USA.
J Nanosci Nanotechnol. 2018 May 1;18(5):3095-3101. doi: 10.1166/jnn.2018.14697.
Shigella sonnei isolate invasion plasmid antigen protein, IpaH, was successfully expressed in recombinant overexpression bacterial system. The soluble expression IpaH was enhanced with molecular chaperon co-expressed environment. Specific aptamer IpaH17 was isolated through the SELEX process and showed fM binding affinity. IpaH17-SPR biosensor platform was involved to verify the binding sensitivity and specificity. The IpaH concentration dependent IpaH17-SPR sensor response was highly linear with a linear regression constant of 99.4% in the range between 0 and 100 ng/mL. In addition, S. sonnei revealed the specific RU value and detected in a real-time manner within 1 hour. Our study indicated that IpaH17-SPR sensor can allow for rapid, sensitive and specific determination of Shigella sonnei virulent factor.
宋内志贺氏菌分离株的侵袭质粒抗原蛋白IpaH在重组过表达细菌系统中成功表达。在分子伴侣共表达环境下，IpaH的可溶性表达得到增强。通过SELEX过程分离出特异性适体IpaH17，其显示出飞摩尔级的结合亲和力。采用IpaH17-SPR生物传感器平台验证结合敏感性和特异性。IpaH浓度依赖性的IpaH17-SPR传感器响应在0至100 ng/mL范围内呈高度线性，线性回归常数为99.4%。此外，宋内志贺氏菌显示出特定的响应单位值，并在1小时内实现实时检测。我们的研究表明，IpaH17-SPR传感器能够快速、灵敏且特异地测定宋内志贺氏菌的毒力因子。
