Bienz Mariann, Kubli Eric
Nature. 1981 Nov 12;294(5837):188-190. doi: 10.1038/294188a0.
Although protein synthesis usually terminates when a stop codon is reached along the messenger RNA sequence, there are examples, mainly in viruses, of the stop codon being suppressed by a tRNA species. A strong candidate for this phenomenon occurs in tobacco mosaic virus (TMV) in the form of two proteins (110K and 160K, of molecular weights 110,000 and 160,000, respectively), sharing an N-terminus sequence, which are translated in vitro from a purified species of viral RNA. We have investigated the identity of the tRNA responsible for production of the 160K protein and show here that it is one of the tyrosine tRNAs. Another tyrosine tRNA, in which the first base of the anticodon is highly modified, does not act as a suppressor, indicating the possible regulatory function of such modifications.
虽然蛋白质合成通常在信使RNA序列上遇到终止密码子时终止，但也有一些例子，主要是在病毒中，终止密码子会被一种tRNA物种抑制。这种现象的一个有力候选者出现在烟草花叶病毒（TMV）中，表现为两种蛋白质（分子量分别为110,000和160,000的110K和160K），它们共享一个N端序列，这些蛋白质是从纯化的病毒RNA物种体外翻译而来的。我们研究了负责产生160K蛋白质的tRNA的身份，在此表明它是酪氨酸tRNA之一。另一种酪氨酸tRNA，其反密码子的第一个碱基经过高度修饰，不起抑制作用，这表明这种修饰可能具有调节功能。
