1,4-萘醌衍生物能有效抑制白色念珠菌的生长，抑制菌丝形成，且对斑马鱼胚胎无毒性。

1,4-Naphthoquinone derivatives potently suppress Candida albicans growth, inhibit formation of hyphae and show no toxicity toward zebrafish embryos.

作者信息

Janeczko Monika, Kubiński Konrad, Martyna Aleksandra, Muzyczka Angelika, Boguszewska-Czubara Anna, Czernik Sławomir, Tokarska-Rodak Małgorzata, Chwedczuk Marta, Demchuk Oleg M, Golczyk Hieronim, Masłyk Maciej

机构信息

Department of Molecular Biology, Faculty of Biotechnology and Environmental Sciences, The John Paul II Catholic University of Lublin, ul. Konstantynów 1i, 20-708 Lublin, Poland.

Department of Medical Chemistry, Medical University of Lublin, ul. Chodźki 4A, 20-093, Lublin, Poland.

出版信息

J Med Microbiol. 2018 Apr;67(4):598-609. doi: 10.1099/jmm.0.000700. Epub 2018 Feb 20.

DOI:10.1099/jmm.0.000700

PMID:29461185

Abstract

PURPOSE

In this study, we applied various assays to find new activities of 1,4-naphthoquinone derivatives for potential anti-Candida albicans applications.

METHODOLOGY

These assays determined (a) the antimicrobial effect on growth/cell multiplication in fungal cultures, (b) the effect on formation of hyphae and biofilm, (c) the influence on cell membrane integrity, (d) the effect on cell morphology using atomic force microscopy, and (e) toxicity against zebrafish embryos. We have demonstrated the activity of these compounds against different Candida species and clinical isolates of C. albicans.

KEY FINDINGS

1,4-Naphthoquinones significantly affected fungal strains at 8-250 mg l of MIC. Interestingly, at concentrations below MICs, the chemicals showed effectiveness in inhibition of hyphal formation and cell aggregation in Candida. Of note, atomic force microscopy (AFM) analysis revealed an influence of the compounds on cell morphological properties. However, at low concentrations (0.8-31.2 mg l), it did not exert any evident toxic effects on zebrafish embryos.

CONCLUSIONS

Our research has evidenced the effectiveness of 1,4-naphthoquinones as potential anti-Candida agents.

摘要

目的

在本研究中，我们应用了多种检测方法来寻找1,4-萘醌衍生物在潜在抗白色念珠菌应用中的新活性。

方法

这些检测方法确定了（a）对真菌培养物中生长/细胞增殖的抗菌作用，（b）对菌丝和生物膜形成的影响，（c）对细胞膜完整性的影响，（d）使用原子力显微镜对细胞形态的影响，以及（e）对斑马鱼胚胎的毒性。我们已经证明了这些化合物对不同念珠菌属物种和白色念珠菌临床分离株的活性。

主要发现

1,4-萘醌在8-250mg/l的最低抑菌浓度（MIC）下对真菌菌株有显著影响。有趣的是，在低于MIC的浓度下，这些化学物质在抑制念珠菌的菌丝形成和细胞聚集方面显示出有效性。值得注意的是，原子力显微镜（AFM）分析揭示了这些化合物对细胞形态特性的影响。然而，在低浓度（0.8-31.2mg/l）下，它对斑马鱼胚胎没有产生任何明显的毒性作用。