Rigvava S, Kusradze I, Karumidze N, Dvalidze T, Katsitadze M, Barbakadze S, Bolkvadze D, Goderdzishvili M
G.Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilisi; Ivane Javakhishvili Tbilisi State University, Georgia.
Georgian Med News. 2018 Jan(274):158-164.
Bacteriophages represent the widest group of viruses, from which only virulent phages are used as antibacterial agent. But the picture in the case of temperate phage is absolutely different; many lysogenic phages express gene products that have subtle effects on the phenotype of the host cell. This process is called lysogenic conversion. In present study we characterized new temperate Enterococcus faecium phage vB_GEC_EFS_2, which was isolated from river Mtkvari. The phage is a member of Siphoviridae family. Whole genome of phage vB_GEC-EfS_2 was sequenced and analyzed. Total length of the genome of phage vB_GEC_EFS_2 is 38 508bp, The assembly contains 65 ORFs, among them - 3 lysis genes , genes coded 13 structural proteins, 1 DNA replication-associated gene, 1 gene coded integration, 3 - lysis-lysogenic cycle regulation, 43 hypothetical proteins. One holin gene contained "Haemolysin XhIA" domain which is surface associated haemolisyn. We isolated and purified holin gene and determine its haemolitic activity alongside with vB_GEC_EfS_2 phage lysate. We clarified the XhIA domain function and role in protein's haemolytic nature and described another kind of lysogenic conversion.
噬菌体是种类最为繁多的病毒群体，其中只有烈性噬菌体被用作抗菌剂。但温和噬菌体的情况则完全不同；许多溶原性噬菌体表达的基因产物会对宿主细胞的表型产生微妙影响。这个过程被称为溶原性转变。在本研究中，我们对从姆特卡里河分离出的新型温和粪肠球菌噬菌体vB_GEC_EFS_2进行了特性分析。该噬菌体属于长尾噬菌体科。对噬菌体vB_GEC-EfS_2的全基因组进行了测序和分析。噬菌体vB_GEC_EFS_2基因组的总长度为38508bp，组装后的基因组包含65个开放阅读框，其中包括3个裂解基因、编码13种结构蛋白的基因、1个与DNA复制相关的基因、1个编码整合的基因、3个裂解-溶原周期调控基因以及43个假定蛋白。一个孔蛋白基因含有“溶血素XhIA”结构域，该结构域是与表面相关的溶血素。我们分离并纯化了孔蛋白基因，并与vB_GEC_EfS_2噬菌体裂解液一起测定了其溶血活性。我们阐明了XhIA结构域在蛋白质溶血特性中的功能和作用，并描述了另一种溶原性转变。
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