Chun-Lian Tang, Zhi-Qin Shen, Jia-Hui Lei, Ming-Sen Jiang, Qiong-Xing Shao, Jin-Song Wang
Department of Clinical Laboratory, Wuchang Hospital, Wuhan 430063, China.
Department of Pathogenic Biology, Tongji Medical College, Huazhong University of Science and Technology, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 May 20;28(3):269-274. doi: 10.16250/j.32.1374.2015150.
To explore the effect and mechanism of CD4CD25 Tregs (Tregs) on the protective efficacy of gluthatione-S-transferase (GST) against in mice.
Female BALB/c mice were divided randomly into five groups：a normal control group, an infected control group, an anti-CD25mAb group, a GST immunization group and a combination group with GST immunization and anti-CD25 mAb. The GST group and combination group were injected percutaneously with GST 50 μg each mouse, the other two groups were injected with equal volume PBS. The immunization was performed for 3 times for two-week interval, and 2 weeks after the last immunization, each mouse was challenged with 40 cercaria. Two weeks post-infection, the combination group and anti-CD25 mAb group were injected intraperitoneally with 300 μg antiCD25 mAb each mouse. The mice were succumbed 2 weeks, 3 weeks, 4 weeks and 5 weeks post-infection respectively. The percentages of CD4CD25 Tregs in splenocytes of mice were measured with flow cytometer. The levels of IFN-γ, IL-2, IL-4, IL-5 and TGF-β in cell cultural supernatants were determined by sandwich-ELISA after stimulation with Con A. The liver sections were stained with hematoxylin and eosin.
The worm burden in the combination group (15.80±2.74) was significantly lower than those of the infected control group (27.78±3.15), anti-CD25 mAb group (21.50±4.21), and GST group (20.84± 6.46). Compared to those of the infected control group, the percentages of CD4CD25 Tregs were significantly higher in the GST group, while the percentages of CD4CD25 Tregs were significantly lower post-anti-CD25 mAb-administration. Regardless of GST administration, the levels of IFN-γ, IL-2, IL-4 and IL-5 after anti-CD25 mAb were significantly higher than those of the infected control groups. There were no significant differences of egg granuloma and the level of TGF-β between each group.
CD4CD25 Tregs could be partially blocked by anti-CD25 mAb while Th1 and Th2 type immunization response could be enhanced, which plays a role in improving the protective efficacy of GST against of .
探讨CD4CD25调节性T细胞（Tregs）对谷胱甘肽 - S - 转移酶（GST）抗小鼠体内[具体感染物未提及]保护效果的影响及机制。
将雌性BALB/c小鼠随机分为五组：正常对照组、感染对照组、抗CD25单克隆抗体组、GST免疫组以及GST免疫与抗CD25单克隆抗体联合组。GST组和联合组每只小鼠经皮注射50μg GST，另外两组注射等体积PBS。每隔两周免疫一次，共免疫3次，末次免疫后2周，每只小鼠感染40只尾蚴。感染后2周，联合组和抗CD25单克隆抗体组每只小鼠腹腔注射300μg抗CD25单克隆抗体。分别在感染后2周、3周、4周和5周处死小鼠。用流式细胞仪检测小鼠脾细胞中CD4CD25 Tregs的百分比。用Con A刺激后，采用夹心ELISA法测定细胞培养上清液中IFN - γ、IL - 2、IL - 4、IL - 5和TGF - β的水平。肝组织切片进行苏木精 - 伊红染色。
联合组的虫负荷（15.80±2.74）显著低于感染对照组（27.78±3.15）、抗CD25单克隆抗体组（21.50±4.21）和GST组（20.84±6.46）。与感染对照组相比，GST组CD4CD25 Tregs的百分比显著升高，而给予抗CD25单克隆抗体后CD4CD25 Tregs的百分比显著降低。无论是否给予GST，抗CD25单克隆抗体处理后IFN - γ、IL - 2、IL - 4和IL - 5的水平均显著高于感染对照组。各组间虫卵肉芽肿和TGF - β水平无显著差异。
抗CD25单克隆抗体可部分阻断CD4CD25 Tregs，同时增强Th1和Th2型免疫反应，这在提高GST抗[具体感染物未提及]的保护效果中发挥作用。
