Boonen Kurt, De Haes Wouter, Van Houtven Joris, Verdonck Rik, Baggerman Geert, Valkenborg Dirk, Schoofs Liliane
Research Group of Functional Genomics and Proteomics, Department of Biology, KU Leuven, Leuven, Belgium.
Research Group of Molecular and Functional Neurobiology, Department of Biology, KU Leuven, Leuven, Belgium.
Methods Mol Biol. 2018;1719:141-159. doi: 10.1007/978-1-4939-7537-2_9.
In differential peptidomics, peptide profiles are compared between biological samples and the resulting expression levels are correlated to a phenotype of interest. This, in turn, allows us insight into how peptides may affect the phenotype of interest. In quantitative differential peptidomics, both label-based and label-free techniques are often employed. Label-based techniques have several advantages over label-free methods, primarily that labels allow for various samples to be pooled prior to liquid chromatography-mass spectrometry (LC-MS) analysis, reducing between-run variation. Here, we detail a method for performing quantitative peptidomics using stable amine-binding isotopic and isobaric tags.
在差异肽组学中，会比较生物样品之间的肽谱，并将所得的表达水平与感兴趣的表型相关联。这进而使我们能够深入了解肽如何影响感兴趣的表型。在定量差异肽组学中，通常会采用基于标记和无标记技术。基于标记的技术相对于无标记方法具有多个优势，主要在于标记允许在液相色谱 - 质谱（LC-MS）分析之前将各种样品合并，从而减少批次间差异。在此，我们详细介绍一种使用稳定胺结合同位素和等压标签进行定量肽组学分析的方法。
