Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Azadi St, Tehran, Iran.
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Azadi St, Tehran, Iran.
J Mycol Med. 2018 Jun;28(2):355-360. doi: 10.1016/j.mycmed.2018.01.005. Epub 2018 Feb 22.
Systemic candidiasis has become an emerging fungal infection in recent years. Anti-Candida resistance to conventional antifungal agents has subsequently increased. This study reported the chemical composition, antioxidant and anti-Candida activity of Origanum majorana, Artemisia dracunculus, Cymbopogon citrate, Cinnamomum verum and Caryophyllus aromaticus essential oils. Different Candida species, from urine tracts of hospitalized patients, were included to be challenged with understudied essential oils. Chemical compositions were determined using gas chromatography/mass spectroscopy (GC/MS) analysis and antioxidant activity was measured using DDPH assay. MIC of these essential oils was evaluated using broth micro-dilution test. Caryophyllus aromaticus had the highest antioxidant activity while the lowest antioxidant activity was for Artemisia dracunculus. MICs of Cinnamomum verum, Caryophillium aromaticus, Artemisia dracunculus, Origanum vulgare and Cymbopogon citratus essential oils ranged from 125 to 175μg/mL (mean value: 147.7±25.5μg/mL), 700 to 1000μg/mL (mean value: 740.9±105.4μg/mL), 1000 to 2000μg/mL (mean value: 1454.5±509.6μg/mL), 173 to 350μg/mL (mean value: 208±55.8μg/mL) and 125 to 175μg/mL (mean value: 156.8±24.6μg/mL) for different Candida species, respectively. In general, natural compounds are suitable to be used as anti-Candida and antioxidant agents. However in this stage, these compounds could be applied as supplementary agents along with conventional antifungal drugs.
近年来，系统性念珠菌病已成为一种新兴的真菌感染。对抗真菌药物的抗药性也随之增加。本研究报告了甜牛至、龙蒿、香茅、肉桂和丁香精油的化学成分、抗氧化和抗念珠菌活性。从住院患者的尿样中分离出不同的念珠菌种，用未充分研究的精油进行挑战。使用气相色谱/质谱（GC/MS）分析确定化学成分，使用 DPPH 测定法测量抗氧化活性。使用肉汤微量稀释试验评估这些精油的 MIC。丁香精油具有最高的抗氧化活性，而龙蒿的抗氧化活性最低。肉桂、丁香、龙蒿、牛至和香茅精油的 MIC 值范围为 125 至 175μg/mL（平均值：147.7±25.5μg/mL）、700 至 1000μg/mL（平均值：740.9±105.4μg/mL）、1000 至 2000μg/mL（平均值：1454.5±509.6μg/mL）、173 至 350μg/mL（平均值：208±55.8μg/mL）和 125 至 175μg/mL（平均值：156.8±24.6μg/mL），分别用于不同的念珠菌种。一般来说，天然化合物适合用作抗念珠菌和抗氧化剂。然而，在现阶段，这些化合物可以作为补充剂与常规抗真菌药物一起使用。
