热休克因子对子宫内膜的影响。
The impact of HSF on endometrium.
作者信息
Bao Hongchu, Wang Guichan, Huang Xin, Wang Meimei, Wang Xinrong, Hao Cuifang
机构信息
Department of Reproductive Medicine, The Affiliated Yantai Yuhuangding Hospital, Qingdao University, Yantai, Shandong, China.
Department of Gynecology and Obstetrics, The Affiliated Yantai Yuhuangding Hospital, Qingdao University, Yantai, Shandong, China.
出版信息
Rev Assoc Med Bras (1992). 2017 Dec;63(12):1069-1075. doi: 10.1590/1806-9282.63.12.1069.
OBJECTIVE
We conducted the research in order to explore the impact of hydrosalpinx fluid (HSF) on endometrium.
METHOD
HSF group: 261 patients with HSF scheduled to undergo laparoscopic surgery 3 to 7 days after menstruation in our center. Hysteroscopy would also be performed in order to observe the endometrial morphology during the surgery. Sixty (60) patients would be randomly selected for endometrial biopsy in order to detect the inflammatory cytokines TNF-a and IL-2 mRNA. Non-HSF group: 210 patients with no evidence of HSF due to chronic salpingitis or pelvic adhesion. IVF-ET treatment was performed after eliminating the factor of male infertility and hysteroscopy was conducted before the treatment. Fifty (50) patients underwent endometrial biopsy in order to detect TNF-a and IL-2 mRNA.
RESULTS
Hysteroscopy was performed in 261 patients with HSF and 210 patients without HSF. The incidence rate of endometritis manifestation among these two groups of patients was 37.2% (97/261) and 20.5% (43/210), respectively. The incidence rate of endometritis in the patients with HSF is significantly higher than in the patients without HSF (p<0.05). Sixty (60) patients from the HSF group and 50 patients from the non-HSF group were regrouped according to inflammatory and normal manifestation after the endometrial biopsy. There were 49 patients in the inflammatory manifestation group and 61 patients in the normal manifestation group. RT-PCR technology was adopted to detect the expression of inflammatory cytokines TNF-a and IL-2 mRNA in endometrial tissue. The level of TNF-a mRNA expression in endometrial tissues with inflammatory manifestation was higher than in normal endometrium (76.75±11.95 vs. 23.45±9.75, p<0.01). There are significant differences between them. The level of IL-2 mRNA expression in endometrial tissues with inflammatory manifestation was higher than that found in normal endometrium (80.56±13.35 vs. 35.12±8.35, p<0.01). There are significant differences between them.
CONCLUSION
Chronic endometritis is related to HSF and may therefore affect endometrial receptivity.
目的
我们开展此项研究以探讨输卵管积水液（HSF）对子宫内膜的影响。
方法
HSF组：261例有HSF的患者计划在月经后3至7天在本中心接受腹腔镜手术。术中还将进行宫腔镜检查以观察子宫内膜形态。随机选择60例患者进行子宫内膜活检以检测炎性细胞因子TNF-α和IL-2 mRNA。非HSF组：210例因慢性输卵管炎或盆腔粘连无HSF证据的患者。在排除男性不育因素后进行体外受精-胚胎移植（IVF-ET）治疗，治疗前进行宫腔镜检查。50例患者进行子宫内膜活检以检测TNF-α和IL-2 mRNA。
结果
对261例有HSF的患者和210例无HSF的患者进行了宫腔镜检查。这两组患者中子宫内膜炎表现的发生率分别为37.2%（97/261）和20.5%（43/210）。有HSF的患者中子宫内膜炎的发生率显著高于无HSF的患者（p<0.05）。子宫内膜活检后，HSF组的60例患者和非HSF组的50例患者根据炎症和正常表现重新分组。炎症表现组有49例患者，正常表现组有61例患者。采用逆转录-聚合酶链反应（RT-PCR）技术检测子宫内膜组织中炎性细胞因子TNF-α和IL-2 mRNA的表达。有炎症表现的子宫内膜组织中TNF-α mRNA表达水平高于正常子宫内膜（76.75±11.95 vs. 23.45±9.75，p<0.01）。两者之间存在显著差异。有炎症表现的子宫内膜组织中IL-2 mRNA表达水平高于正常子宫内膜（80.56±13.35 vs. 35.12±8.35，p<0.01）。两者之间存在显著差异。
结论
慢性子宫内膜炎与HSF有关，因此可能影响子宫内膜容受性。
Zotero 插件