Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany.
Department of Bioprocess Engineering-Microbiology, Hannover University of Applied Science and Arts, 30453 Hannover, Germany.
J Dairy Sci. 2018 May;101(5):4317-4324. doi: 10.3168/jds.2017-14100. Epub 2018 Mar 1.
Fast and accurate identification of disease-causing pathogens is essential for specific antimicrobial therapy in human and veterinary medicine. In these experiments, dogs were trained to identify Staphylococcus aureus and differentiate it from other common mastitis-causing pathogens by smell. Headspaces from agar plates, inoculated raw milk samples, or field samples collected from cows with Staphylococcus aureus and other mastitis-causing pathogens were used for training and testing. The ability to learn the specific odor of Staphylococcus aureus in milk depended on the concentration of the pathogens in the training samples. Sensitivity and specificity for identifying Staphylococcus aureus were 91.3 and 97.9%, respectively, for pathogens grown on agar plates; 83.8 and 98.0% for pathogens inoculated in raw milk; and 59.0 and 93.2% for milk samples from mastitic cows. The results of these experiments underline the potential of odor detection as a diagnostic tool for pathogen diagnosis.
快速准确地鉴定致病病原体对于人类和兽医医学中的特定抗菌治疗至关重要。在这些实验中，通过嗅觉训练狗识别金黄色葡萄球菌并将其与其他常见乳腺炎病原体区分开来。使用来自接种琼脂平板的生奶样本或从患有金黄色葡萄球菌和其他乳腺炎病原体的奶牛采集的现场样本的顶空部分进行训练和测试。在牛奶中学习金黄色葡萄球菌特定气味的能力取决于训练样本中病原体的浓度。在琼脂平板上培养的病原体的识别金黄色葡萄球菌的灵敏度和特异性分别为 91.3%和 97.9%；接种生奶中的病原体的灵敏度和特异性分别为 83.8%和 98.0%；乳腺炎奶牛奶样的灵敏度和特异性分别为 59.0%和 93.2%。这些实验的结果强调了气味检测作为病原体诊断的诊断工具的潜力。
