Mukherji B, Guha A, Loomis R, Ergin M T
J Immunol. 1987 Mar 15;138(6):1987-91.
The cytotoxic host immune response toward autologous human cancer may be regulated by the immunoregulatory network. Here we show that helper T cells, cloned from peripheral blood lymphocytes that were sensitized in vitro against an autologous human malignant paraganglioma, proliferated against and made interleukin 2 when cocultured with the tumor-associated antigen in the presence of autologous accessory cells. Furthermore, the helper cell clones amplified cytotoxic immune response by peripheral blood lymphocytes against the paraganglioma cells in coculture with the blood lymphocytes and the paraganglioma cells. An autologous T cell line bearing suppressor phenotype, established from a lymph node that had been infiltrated with the paraganglioma tumor cells, in contrast to the helper cells, selectively suppressed the cytotoxic immune response by the blood lymphocytes against the paraganglioma cells in identical coculture. These results, therefore, demonstrate the existence of cell-mediated immunologic regulations of the cytotoxic immune response (concurrent amplification and suppression in the same host) against an autologous human tumor.
针对自体人类癌症的细胞毒性宿主免疫反应可能受免疫调节网络调控。在此我们表明，从体外致敏于自体人类恶性副神经节瘤的外周血淋巴细胞中克隆出的辅助性T细胞，在与肿瘤相关抗原共同培养且存在自体辅助细胞的情况下，会针对该抗原增殖并产生白细胞介素2。此外，辅助性T细胞克隆在与血淋巴细胞和副神经节瘤细胞共同培养时，增强了外周血淋巴细胞针对副神经节瘤细胞的细胞毒性免疫反应。相比之下，从被副神经节瘤肿瘤细胞浸润的淋巴结建立的具有抑制表型的自体T细胞系，会选择性抑制相同共同培养体系中血淋巴细胞针对副神经节瘤细胞的细胞毒性免疫反应。因此，这些结果证明了针对自体人类肿瘤的细胞毒性免疫反应存在细胞介导的免疫调节（在同一宿主中同时存在增强和抑制）。
