NF-κB 信号通路介导的脂联素基因在 2 型糖尿病发病机制中的作用。

The role of adiponectin gene mediated by NF-κB signaling pathway in the pathogenesis of type 2 diabetes.

机构信息

Emergency Department, Third Xiangya Hospital of Central South University, Changsha, Hunan Province, Xiangya, China.

出版信息

Eur Rev Med Pharmacol Sci. 2018 Feb;22(4):1106-1112. doi: 10.26355/eurrev_201802_14398.

DOI:10.26355/eurrev_201802_14398

PMID:29509263

Abstract

OBJECTIVE

To investigate the role of adiponectin (ADPN) gene mediated by NF-κB signaling pathway in the pathogenesis of type 2 diabetes (T2DM).

PATIENTS AND METHODS

The levels of expression of ADPN were measured by fluorescence quantitative PCR technology, enzyme-linked immunosorbent assay, Western-blotting and immunohistochemistry in 78 patients with type 2 diabetes.

RESULTS

Compared with the normal population, the levels of expression of ADPN and NF-κB mRNA in the blood of patients with type 2 diabetes were significantly reduced (p < 0.05). The detection of ADPN gene protein expression in normal population and patients with type 2 diabetes by enzyme-linked immunosorbent assay showed that ADPN gene protein expression in type 2 diabetic patients (1.26 ± 0.73) µg/l was significantly lower (p < 0.05) than ADPN gene protein expression of the normal population (3.26 ± 1.25) µg/l. At the same time, the detection of the expression of NF-κB gene showed that the protein expression in type 2 diabetic patients (0.58 ± 0.15) µg/l was significantly lower (p < 0.05) than that in the normal population (1.67 ± 1.04) µg/l. The results of Western-blotting were consistent with the results of enzyme-linked immunosorbent assay. Immunohistochemical results also showed that the percentage of ADPN positive cells in patients with type 2 diabetes (25.47%) was significantly lower (p < 0.05) than that in the normal population (79.47%).

CONCLUSIONS

ADPN gene in the human body can be involved in the pathogenesis of T2DM through the NF-κB signaling pathway.

摘要

目的

探讨 NF-κB 信号通路介导的脂联素（ADPN）基因在 2 型糖尿病（T2DM）发病机制中的作用。

方法

采用荧光定量 PCR 技术、酶联免疫吸附试验、Western-blotting 和免疫组织化学方法检测 78 例 2 型糖尿病患者 ADPN 的表达水平。

结果

与正常人群相比，2 型糖尿病患者血液中 ADPN 和 NF-κB mRNA 的表达水平明显降低（p＜0.05）。通过酶联免疫吸附试验检测正常人群和 2 型糖尿病患者 ADPN 基因蛋白的表达情况，结果显示 2 型糖尿病患者 ADPN 基因蛋白表达（1.26±0.73）μg/L 明显低于正常人群 ADPN 基因蛋白表达（3.26±1.25）μg/L（p＜0.05）。同时，NF-κB 基因表达的检测显示，2 型糖尿病患者的蛋白表达（0.58±0.15）μg/L 明显低于正常人群（1.67±1.04）μg/L（p＜0.05）。Western-blotting 的结果与酶联免疫吸附试验的结果一致。免疫组化结果也显示，2 型糖尿病患者 ADPN 阳性细胞的百分比（25.47%）明显低于正常人群（79.47%）（p＜0.05）。