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[从地理上相距遥远地区的土壤和水中分离出的耐热性石油降解细菌]

[Thermotolerant oil-degrading bacteria isolated from soil and water of geographically distant regions].

作者信息

Delegan Ya A, Vetrova A A, Akimov V N, Titok M A, Filonov A E, Boronin A M

出版信息

Prikl Biokhim Mikrobiol. 2016 Jul-Aug;52(4):383-91.

PMID:29512982
Abstract

Oil-degrading bacteria were isolated from soil and water samples taken in Russia, Kazakhstan, and the Antarctic; 13 of 86 strains proved to be thermotolerant. These bacteria utilized crude oil at 45–50°C; their growth optimum (35–37°C) and range (20–53°C) differ from those of mesophilic bacteria. Thermotolerant strains were identified as representatives of the genera Rhodococcus and Gordonia. It was shown that their ability to degrade petroleum products does not differ at 24 and 45°C. The strains Rhodococcus sp. Par7 and Gordonia sp. 1D utilized 14 and 20% of the oil, respectively, in 14 days at 45°C. All of the isolated thermotolerant bacteria grew in a medium containing 3% NaCl; the medium for the strains Gordonia amicalis 1B and Gordonia sp. 1D contained up to 10% NaCl. The bacteria G. amicalis and Rhodococcus erythropolis were able to utilize crude oil and individual hydrocarbons at higher (up to 50°C) temperatures.

摘要

从俄罗斯、哈萨克斯坦和南极洲采集的土壤和水样中分离出了石油降解菌;86株菌株中有13株被证明具有耐热性。这些细菌能在45 - 50°C的温度下利用原油;它们的最适生长温度(35 - 37°C)和生长温度范围(20 - 53°C)与嗜温细菌不同。耐热菌株被鉴定为红球菌属和戈登氏菌属的代表。结果表明,它们在24°C和45°C下降解石油产品的能力没有差异。红球菌属菌株Par7和戈登氏菌属菌株1D在45°C下14天内分别利用了14%和20%的石油。所有分离出的耐热细菌都能在含有3%氯化钠的培养基中生长;友好戈登氏菌1B和戈登氏菌属菌株1D的培养基中氯化钠含量高达10%。友好戈登氏菌和红平红球菌能够在较高温度(高达50°C)下利用原油和个别碳氢化合物。

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