Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany; Chair for Biological Imaging, Technical University of Munich, Munich, Germany.
Chair of Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, Germany; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany; ZIEL - Institute for Food and Health, Technical University of Munich, Freising, Germany.
Cell Metab. 2018 Mar 6;27(3):689-701.e4. doi: 10.1016/j.cmet.2018.02.002.
Metabolism is a fundamental process of life. However, non-invasive measurement of local tissue metabolism is limited today by a deficiency in adequate tools for in vivo observations. We designed a multi-modular platform that explored the relation between local tissue oxygen consumption, determined by label-free optoacoustic measurements of hemoglobin, and concurrent indirect calorimetry obtained during metabolic activation of brown adipose tissue (BAT). By studying mice and humans, we show how video-rate handheld multi-spectral optoacoustic tomography (MSOT) in the 700-970 nm spectral range enables non-invasive imaging of BAT activation, consistent with positron emission tomography findings. Moreover, we observe BAT composition differences between healthy and diabetic tissues. The study consolidates hemoglobin as a principal label-free biomarker for longitudinal non-invasive imaging of BAT morphology and bioenergetics in situ. We also resolve water and fat components in volunteers, and contrast MSOT readouts with magnetic resonance imaging data.
新陈代谢是生命的基本过程。然而，目前由于缺乏用于体内观察的适当工具，对局部组织代谢的非侵入性测量受到限制。我们设计了一个多模块平台，探索了局部组织耗氧量与通过血红蛋白无标记光声测量确定的关系，以及在棕色脂肪组织（BAT）代谢激活过程中同时获得的间接量热法之间的关系。通过对小鼠和人类的研究，我们展示了在 700-970nm 光谱范围内的视频速率手持式多光谱光声断层扫描（MSOT）如何能够对 BAT 激活进行非侵入性成像，这与正电子发射断层扫描的结果一致。此外，我们还观察到健康组织和糖尿病组织之间的 BAT 组成差异。该研究将血红蛋白作为一种主要的无标记生物标志物，用于对 BAT 形态和生物能量学进行纵向非侵入性原位成像。我们还在志愿者中解析了水和脂肪成分，并将 MSOT 读数与磁共振成像数据进行对比。
