Gamngoen Ratikorn, Putim Chanyanuch, Salee Parichat, Phunpae Ponrut, Butr-Indr Bordin
Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Tuberculosis (Edinb). 2018 Jan;108:64-69. doi: 10.1016/j.tube.2017.11.002. Epub 2017 Nov 4.
Drug resistance to Mycobacterium tuberculosis is a major health problem worldwide. Mycobacterium tuberculosis can progress to be mono-drug resistant or multi-drug resistant by improper treatment. The chemical stress of M. tuberculosis was performed in this study. Rv0559c is an unknown secreted protein. Rv0560c is a putative benzoquinone methyltransferase of M. tuberculosis cell. Rv0559c gene is located downstream of Rv0560c gene. Both genes respond to salicylate stress. Drug susceptible, isoniazid resistant, rifampicin resistant and multi-drug resistant phenotypes of M. tuberculosis clinical isolates were used to determine the expression of Rv0559c and Rv0560c by qRT-PCR. In all of mycobacteria strains there was up-regulation in both genes when stressed with isoniazid. This study determined the expression of both genes, which may play important roles in the drug resistance mechanism of mycobacteria.
结核分枝杆菌耐药性是全球主要的健康问题。结核分枝杆菌通过不当治疗可能会发展为单药耐药或多药耐药。本研究对结核分枝杆菌进行了化学应激实验。Rv0559c是一种未知的分泌蛋白。Rv0560c是结核分枝杆菌细胞中一种假定的苯醌甲基转移酶。Rv0559c基因位于Rv0560c基因的下游。这两个基因均对水杨酸盐应激有反应。利用结核分枝杆菌临床分离株的药物敏感、异烟肼耐药、利福平耐药和多药耐药表型，通过qRT-PCR测定Rv0559c和Rv0560c的表达。在所有分枝杆菌菌株中，用异烟肼应激时这两个基因均上调。本研究确定了这两个基因的表达，它们可能在分枝杆菌的耐药机制中发挥重要作用。
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