Boychuk T M, Kmet T I
Fiziol Zh (1994). 2016;62(2):72-8. doi: 10.15407/fz62.02.072.
The effect of diabetes mellitus on the dynamics of neurocyte and gliacyte apoptosis intensity in the cortex of the frontal, parietal and temporal lobes of the cerebral hemispheres under conditions of ischemic-reperfusion lesion has been studied in experiments on rats. The level of apoptotic processes in the neuro- and gliacytes of the frontal cortex has been found to be unchanged after 20 minutes of carotid ischemia followed by one hour reperfusion according to the indices examined in animals with out diabetes mellitus. Apoptosis of neurocytes is activated in the cortex of the parietal lobe, and that of the neuro- and gliacytes – in the cortex of the temporal lobe. Three-month diabetes mellitus intensifies apoptosis of neurons and glial cells in the cortex of the frontal and temporal lobes, neurons in the cortex of the parietal lobe and decreases apoptosis of gliacytes in it. In early ischemic-reperfusion period the activity of apoptotic processes in the cortex of the frontal and temporal lobes does not change in animals with diabetes mellitus, but it decreases in the cortex of the parietal lobe at the expense of glial cells. On the 12th day of observation the activity of apoptotic processes in neurocytes of the cortex of the temporal lobe increases in rats without diabetes mellitus, and it decreases in the glial cells. We detected a reduced content of the protein p53 in neurons and increased density of р53+-cells. In this period of observation in rats with diabetes mellitus the activity of apoptotic processes decreases in general both in neurons and glial cells of all the lobes. The results obtained point for the availability of regional differences in the dynamics of reaction of the cerebral hemisphere lobes in response to ischemic-reperfusion injury charachterized by the intensity of apoptosis of neurons and glial cells. The results also point for modifying effect of diabetes mellitus on the indices studied.
在大鼠实验中，研究了糖尿病对脑缺血再灌注损伤条件下大脑半球额叶、顶叶和颞叶皮质神经细胞和神经胶质细胞凋亡强度动态变化的影响。根据对无糖尿病动物检测的指标，在颈动脉缺血20分钟后再灌注1小时，额叶皮质神经细胞和神经胶质细胞的凋亡水平未发生变化。顶叶皮质神经细胞凋亡被激活，颞叶皮质神经细胞和神经胶质细胞凋亡被激活。三个月的糖尿病会加剧额叶和颞叶皮质神经元和神经胶质细胞的凋亡、顶叶皮质神经元的凋亡，并减少其中神经胶质细胞的凋亡。在早期缺血再灌注期，糖尿病动物额叶和颞叶皮质的凋亡过程活性没有变化，但顶叶皮质的凋亡过程活性以神经胶质细胞为代价而降低。在观察的第12天，无糖尿病大鼠颞叶皮质神经细胞的凋亡过程活性增加，而神经胶质细胞的凋亡过程活性降低。我们检测到神经元中蛋白质p53含量降低，p53+细胞密度增加。在观察的这一时期，糖尿病大鼠所有脑叶的神经元和神经胶质细胞的凋亡过程活性总体上均降低。所获得的结果表明，大脑半球各叶对缺血再灌注损伤反应的动态变化存在区域差异，其特征为神经元和神经胶质细胞的凋亡强度。结果还表明糖尿病对所研究指标有调节作用。
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