内皮素-1及其受体ET与药物性牙龈增生中的ET

Endothelin-1 and Its Receptors ET and ET in Drug-Induced Gingival Overgrowth.

作者信息

Tamilselvan S, Raju Surya Narayana, Loganathan D, Kamatchiammal S, Abraham Georgie, Suresh R

机构信息

Department of Periodontics, Sri Ramachandra Dental College and Hospital, Sri Ramachandra Medical College and Research Institute, Deemed University, Porur, Chennai, Tamilnadu, India.

National Environmental Engineering Research Institute, Council of Scientific and Industrial Research, Taramani, Chennai, Tamilnadu, India.

出版信息

J Periodontol. 2007 Feb;78(2):290-295. doi: 10.1902/jop.2007.060172.

DOI:10.1902/jop.2007.060172

PMID:29539178

Abstract

BACKGROUND

The purpose of this study was to study the expression of endothelin-1 (ET-1) and its receptors ET and ET in normal human gingiva and cyclosporin-induced gingival fibroblasts.

METHODS

Gingival samples were collected from eight normal healthy individuals, eight patients with periodontitis, and eight patients with cyclosporin A (CsA)-induced gingival overgrowth. Total RNA was extracted from tissue samples, and reverse transcriptase-polymerase chain reaction was performed for ET-1, ET , and ET . ET-1 protein was estimated from the tissues by enzyme-linked immunosorbent assay. The expression of ET-1 and its receptors was also examined in gingival fibroblast cells treated with CsA.

RESULTS

ET-1 mRNA expression was significantly higher in patients with CsA-induced gingival overgrowth (P <0.001) than in patients with periodontitis and the controls. ET mRNA was expressed more than the ET in all examined samples. In human gingival fibroblasts, ET-1 expression was increased with CsA incorporation compared to controls (P <0.001).