James S P, Graeff A S, Zeitz M
Cell Immunol. 1987 Jul;107(2):372-83. doi: 10.1016/0008-8749(87)90245-0.
To define the characteristics of T cells associated with the gastrointestinal tract, the phenotypes and immunoregulatory function of T cells from mesenteric lymph node (MLN) and lamina propria lymphocytes (LPL) were compared to peripheral blood (PBL) and spleen lymphocytes in normal nonhuman primates. Mesenteric lymph node lymphocytes were characterized by a higher proportion of Leu-3+(CD4+) and 9.3+(alpha-Tp44) lymphocytes and a lower proportion of Leu-2+(CD8) lymphocytes than lymphocytes in other sites. LPL and MLN lymphocytes were both characterized by a higher proportion of cells having the helper-inducer phenotypes (Leu-3+, Leu-8+, Leu-3+, 2H4+) compared to PBL. A lower proportion of cells with the suppressor-inducer phenotypes (Leu-3+, Leu-8+, Leu-3+, 2H4+) was found in LPL, but not in MLN lymphocytes compared to PBL. In studies of the Leu-2+ T cells, it was found that whereas PBL, spleen, and LPL contained approximately equal proportions of Leu-2+, Leu-15+ (suppressor phenotype) and Leu-2+, 9.3+ lymphocytes (cytolytic T-cell phenotype), the MLN T cells were predominantly Leu-2+, 9.3+. Furthermore, the Leu-3/Leu-2 ratio was significantly higher in MLN compared to other sites. In pokeweed mitogen-stimulated cultures, the highest helper function for Ig synthesis was found in MLN. Cells from none of the sites studied showed evidence of increased suppressor cell activity. These results show that MLN and LPL T cells in normal nonhuman primates differ from T cells in peripheral blood and spleen. While both MLN and LPL have a high proportion of T cells with the helper-inducer phenotype, cells with the suppressor-effector phenotype are infrequent in MLN, while cells with the suppressor-inducer phenotype are infrequent in LPL.
为了明确与胃肠道相关的T细胞特征，在正常非人灵长类动物中，将肠系膜淋巴结（MLN）和固有层淋巴细胞（LPL）中的T细胞表型及免疫调节功能与外周血（PBL）和脾淋巴细胞进行了比较。肠系膜淋巴结淋巴细胞的特征在于，与其他部位的淋巴细胞相比，Leu-3 +（CD4 +）和9.3 +（α-Tp44）淋巴细胞比例更高，而Leu-2 +（CD8）淋巴细胞比例更低。与PBL相比，LPL和MLN淋巴细胞均具有较高比例的辅助诱导型表型（Leu-3 +、Leu-8 +、Leu-3 +、2H4 +）细胞。与PBL相比，LPL中具有抑制诱导型表型（Leu-3 +、Leu-8 +、Leu-3 +、2H4 +）的细胞比例较低，但MLN淋巴细胞中并非如此。在对Leu-2 + T细胞的研究中发现，PBL、脾脏和LPL中Leu-2 +、Leu-15 +（抑制型表型）和Leu-2 +、9.3 +淋巴细胞（溶细胞性T细胞表型）的比例大致相等，而MLN T细胞主要为Leu-2 +、9.3 +。此外，与其他部位相比，MLN中的Leu-3 / Leu-2比值显著更高。在商陆丝裂原刺激的培养物中，MLN对Ig合成的辅助功能最高。在所研究的任何部位的细胞中均未显示出抑制细胞活性增强的迹象。这些结果表明，正常非人灵长类动物中的MLN和LPL T细胞与外周血和脾脏中的T细胞不同。虽然MLN和LPL中具有辅助诱导型表型的T细胞比例都很高，但具有抑制效应型表型的细胞在MLN中很少见，而具有抑制诱导型表型的细胞在LPL中很少见。
