肌醇转运体HMIT和SMIT1在耳蜗血管纹中的差异定位。

Differential localizations of the myo-inositol transporters HMIT and SMIT1 in the cochlear stria vascularis.

作者信息

Edamatsu Midori, Kondo Yasuhiro, Ando Motonori

机构信息

Laboratory of Cell Physiology, Department of Science Education, Graduate School of Education, Okayama University, Okayama 700-8530, Japan; Laboratory of Animal Physiology and Pharmacology, Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.

Laboratory of Animal Physiology and Pharmacology, Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.

出版信息

Neurosci Lett. 2018 May 1;674:88-93. doi: 10.1016/j.neulet.2018.03.028. Epub 2018 Mar 15.

DOI:10.1016/j.neulet.2018.03.028

PMID:29551423

Abstract

The cochlear stria vascularis produces endolymph and thereby plays an active role in inner ear homeostasis. We recently reported that the H/myo-inositol cotransporter (HMIT) gene is expressed in the stria vascularis. Here, we examined the protein localization of HMIT and Na/myo-inositol cotransporter 1 (SMIT1) in the stria vascularis by immunohistochemistry. HMIT and SMIT1 were detected in the lateral wall of the cochlear duct. HMIT was widely detected throughout the stria vascularis, while SMIT1 was enriched in the strial basal cells. To examine the localization of HMIT in the stria vascularis in more detail, dissociated strial cells were immunostained, which resulted in the detection of HMIT immunoreactivity in marginal cells. These results indicate that HMIT is expressed in marginal cells and basal cells of the stria vascularis, while SMIT1 expression is enriched in basal cells. We speculate that HMIT and SMIT1 may play important roles in the homeostasis of cochlear fluids, for example by participating in pH regulation and osmoregulation.

摘要

耳蜗血管纹产生内淋巴，从而在内耳内环境稳定中发挥积极作用。我们最近报道，H/肌醇共转运体（HMIT）基因在血管纹中表达。在此，我们通过免疫组织化学检查了HMIT和钠/肌醇共转运体1（SMIT1）在血管纹中的蛋白定位。在耳蜗管的侧壁检测到HMIT和SMIT1。HMIT在整个血管纹中广泛检测到，而SMIT1在血管纹基底细胞中富集。为了更详细地检查HMIT在血管纹中的定位，对解离的血管纹细胞进行免疫染色，结果在边缘细胞中检测到HMIT免疫反应性。这些结果表明，HMIT在血管纹的边缘细胞和基底细胞中表达，而SMIT1表达在基底细胞中富集。我们推测，HMIT和SMIT1可能在耳蜗内液的内环境稳定中发挥重要作用，例如通过参与pH调节和渗透压调节。