毛细管电泳研究抗体-抗原相互作用：综述。

Studies of antibody-antigen interactions by capillary electrophoresis: A review.

机构信息

Chemistry Department, University of Nebraska, Kearney, NE, United States.

出版信息

Methods. 2018 Aug 15;146:66-75. doi: 10.1016/j.ymeth.2018.03.006. Epub 2018 Mar 15.

DOI:10.1016/j.ymeth.2018.03.006

Abstract

Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

摘要

抗体-抗原相互作用在免疫分析的发展中至关重要，可以决定检测限和分析时间。毛细管电泳（CE）是一种强大的技术，可用于定量抗体-抗原相互作用。这些 CE 方法的范围从简单地分离预先混合的抗体和抗原样品作为短塞来允许分离复杂的、游离的抗体和游离的抗原到更复杂的系统，在抗体或抗原存在下注入复合物样品；甚至可以顺序注入抗体和抗原。本综述的目的是确定和描述已用于研究抗体-抗原相互作用的各种 CE 技术。还简要讨论了线性和非线性曲线拟合。