Park Minhee, Hwang Ho Kyoung, Yun Mijin, Lee Woo Jung, Kim Hoguen, Kang Chang Moo
Departments of Pathology and BK21 PLUS for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
Department of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Yonsei University College of Medicine, Seoul, Korea.
Oncotarget. 2018 Jan 3;9(15):12009-12019. doi: 10.18632/oncotarget.23846. eCollection 2018 Feb 23.
We aimed to investigate the metabolic characteristics of Solid pseudopapillary neoplasms (SPNs) in relation signal intensities on F-FDG PET scans.
SPNs of the pancreas commonly show high uptake of 18F-FDG. However, the metabolic characteristics underlying the high F-FDG uptake in SPNs are not well characterized.
mRNA expressions for glucose metabolism were analyzed in five SPNs, five pancreatic ductal adenocarcinomas (PCAs), and paired normal pancreatic tissues. Among the proteins involved in glucose metabolism, the expressions of five proteins (GLUT1, HK1, PFKM, ENO2, and PKM2) were evaluated in 36 SPNs by immunohistochemistry. Clinical patterns of SPN on PET scans were classified according to the proportion of F-FDG uptake within the whole tumor volume (hot: ≥ 70%, mixed: 30 ≤ < 70, and defective: < 30%). PET-based parameters, including maximum standardized uptake value (SUV) and metabolic tumor volume (TMV), were evaluated.
Hot ( = 19), mixed ( = 5), and defective ( = 12) F-FDG uptake patterns were noted in the 36 patients. Radiologic tumor size and SUV differed significantly according to these patterns (ANOVA, < 0.05). GLUT1, HK1, PFKM, ENO2, and PKM2 were highly expressed in SPNs at both the mRNA and protein levels. Defective type SPNs showed lower expression of HK1 ( = 0.014), PKM2 ( = 0.028), and Ki-67 ( = 0.070) with frequent intra-tumoral necrosis ( = 0.007). High Ki-67 expression (≥ 3%) was associated with high SUV in pancreatic SPNs ( = 0.002).
SPN cells harbor an active molecular capacity for increased glucose metabolism. Especially, defective type SPNs were associated with low metabolic activity and related to low Ki-67 index.
我们旨在研究实性假乳头状肿瘤（SPN）在F-FDG PET扫描上的代谢特征与信号强度之间的关系。
胰腺SPN通常显示出18F-FDG的高摄取。然而，SPN中F-FDG高摄取背后的代谢特征尚未得到充分表征。
分析了5例SPN、5例胰腺导管腺癌（PCA）及配对的正常胰腺组织中葡萄糖代谢的mRNA表达。在参与葡萄糖代谢的蛋白质中，通过免疫组织化学评估了36例SPN中5种蛋白质（GLUT1、HK1、PFKM、ENO2和PKM2）的表达。根据整个肿瘤体积内F-FDG摄取的比例对PET扫描上SPN的临床模式进行分类（热型：≥70%，混合型：30%≤<70%，缺损型：<30%）。评估了基于PET的参数，包括最大标准化摄取值（SUV）和代谢肿瘤体积（TMV）。
36例患者中观察到热型（n = 19）、混合型（n = 5）和缺损型（n = 12）F-FDG摄取模式。根据这些模式，放射学肿瘤大小和SUV有显著差异（方差分析，P < 0.05）。GLUT1、HK1、PFKM、ENO2和PKM2在SPN的mRNA和蛋白质水平均高表达。缺损型SPN显示HK1（P = 0.014）、PKM2（P = 0.028）和Ki-67（P = 0.070）表达较低，且肿瘤内坏死频繁（P = 0.007）。高Ki-67表达（≥3%）与胰腺SPN的高SUV相关（P = 0.002）。
SPN细胞具有增加葡萄糖代谢的活跃分子能力。特别是，缺损型SPN与低代谢活性相关，并与低Ki-67指数有关。
