宫颈癌患者原发肿瘤细胞对化疗联合方案的敏感性研究。

Study on the sensitivity of primary tumor cells of patients with cervical cancer to chemotherapeutic combinations.

作者信息

Qiu Fang, Zhao Xia

机构信息

Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, China.

出版信息

J BUON. 2018 Jan-Feb;23(1):117-123.

PMID:29552770

Abstract

PURPOSE

To study the sensitivity of primary tumor cells from patients with cervical cancer to frequently used chemotherapeutic combinations.

METHODS

54 samples from cervical cancer patients were collected and used to develop cell culture in vitro. Eight frequently used chemotherapeutic combinations (taxol, taxol+cisplatin, taxol+carboplatin, topotecan+cisplatin, gemcitabine+cisplatin, docetaxel, gemcitabine, cisplatin+c yclophosphamide+doxorubicin) were prepared in different concentrations [200,100,50,25, and 12.5% plasma peak concentration (PPC)] and added to tumor cell culture. Adenosine triphosphate (ATP) bioluminescence assay was used to quantify cell cytotoxicity.

RESULTS

Sensitivity of primary tumor cell from patients with cervical cancer to 8 chemotherapeutic combinations were: gemcitabine < docetaxel < topotecan+cisplatin < cisplatin +cyclophosphamide+doxorubicin < gemcitabine+cisplatin < taxol < taxol+carboplatin < taxol+cisplatin.

CONCLUSION

Using the drug sensitivity test to assess chemotherapeutics in the treatment of cervical cancer patients offers many benefits, being a first step to a clinically individualized chemotherapy.

摘要

目的

研究宫颈癌患者原发性肿瘤细胞对常用化疗联合方案的敏感性。

方法

收集54例宫颈癌患者的样本，用于体外细胞培养。制备8种常用化疗联合方案（紫杉醇、紫杉醇+顺铂、紫杉醇+卡铂、拓扑替康+顺铂、吉西他滨+顺铂、多西他赛、吉西他滨、顺铂+环磷酰胺+阿霉素）的不同浓度（200、100、50、25和12.5%血浆峰浓度[PPC]），并添加到肿瘤细胞培养物中。采用三磷酸腺苷（ATP）生物发光法测定细胞毒性。