Beijing Key Laboratory of Lignocellulosic Chemistry , Beijing Forestry University , Beijing 100083 , China.
Laboratory of Tree Cell Biology, Graduate School of Agriculture , Kyoto University , Kyoto , 606-8502 , Japan.
J Agric Food Chem. 2018 Apr 4;66(13):3449-3456. doi: 10.1021/acs.jafc.8b00320. Epub 2018 Mar 23.
As the main hemicellulose of poplar, the interaction of xylan with lignin was expected to have profound effect on biomass recalcitrance. In this paper, the dynamic changes of xylan and lignin in poplar cell wall during a mild pretreatment using γ-valerolactone (GVL) was investigated using chemical and microscopic techniques. Synergetic dissolution of branched xylan and lignin from the secondary wall of the fiber cell was found to play a major role in opening the cell wall structure for enzymatic attack. In the case of the removal of xylan and lignin reaching a certain level, β-O-4' cleavage of lignin which destroyed its interaction with hydrophobic cellulose face was found to make great contribution to the enhanced enzymatic hydrolysis. The deep understanding of this process could lead to a new insight into the understanding of the plant cell wall architecture and provide basic information for biomass processing.
作为杨树的主要半纤维素，木聚糖与木质素的相互作用预计会对生物质的抗降解性产生深远的影响。在本文中，使用γ-戊内酯（GVL）对杨树细胞壁进行温和预处理，使用化学和微观技术研究了木聚糖和木质素在细胞壁中的动态变化。发现从纤维细胞壁的次生壁中协同溶解支链木聚糖和木质素，在打开细胞壁结构以进行酶攻击方面发挥了主要作用。在达到一定水平的木聚糖和木质素去除的情况下，木质素的β-O-4'断裂破坏了其与疏水性纤维素表面的相互作用，这对提高酶解效率做出了巨大贡献。对这一过程的深入了解可以为理解植物细胞壁结构提供新的认识，并为生物质加工提供基础信息。
