软脂酸（16:1n7）增加白色脂肪细胞的耗氧量、脂肪酸氧化和 ATP 含量。
Palmitoleic acid (16:1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes.
机构信息
Department of Biological Sciences, Institute of Environmental Sciences, Chemical and Pharmaceutical, Federal University of São Paulo, 210, Sao Nicolau St, Diadema, 09913-030, Brazil.
Department of Nursing , Health Sciences Center, Federal University of Espírito Santo, Vitória, Brazil.
出版信息
Lipids Health Dis. 2018 Mar 20;17(1):55. doi: 10.1186/s12944-018-0710-z.
BACKGROUND
We have recently demonstrated that palmitoleic acid (16:1n7) increases lipolysis, glucose uptake and glucose utilization for energy production in white adipose cells. In the present study, we tested the hypothesis that palmitoleic acid modulates bioenergetic activity in white adipocytes.
METHODS
For this, 3 T3-L1 pre-adipocytes were differentiated into mature adipocytes in the presence (or absence) of palmitic (16:0) or palmitoleic (16:1n7) acid at 100 or 200 μM. The following parameters were evaluated: lipolysis, lipogenesis, fatty acid (FA) oxidation, ATP content, oxygen consumption, mitochondrial mass, citrate synthase activity and protein content of mitochondrial oxidative phosphorylation (OXPHOS) complexes.
RESULTS
Treatment with 16:1n7 during 9 days raised basal and isoproterenol-stimulated lipolysis, FA incorporation into triacylglycerol (TAG), FA oxidation, oxygen consumption, protein expression of subunits representing OXPHOS complex II, III, and V and intracellular ATP content. These effects were not observed in adipocytes treated with 16:0.
CONCLUSIONS
Palmitoleic acid, by concerted action on lipolysis, FA esterification, mitochondrial FA oxidation, oxygen consumption and ATP content, does enhance white adipocyte energy expenditure and may act as local hormone.
背景
我们最近的研究表明，棕榈油酸（16:1n7）可增加白色脂肪细胞中的脂肪分解、葡萄糖摄取和葡萄糖利用以产生能量。在本研究中，我们检验了棕榈油酸是否能调节白色脂肪细胞的生物能量活性这一假设。
方法
为此，在存在（或不存在）100 或 200μM 棕榈酸（16:0）或棕榈油酸（16:1n7）的情况下，将 3T3-L1 前体脂肪细胞分化为成熟脂肪细胞。评估了以下参数：脂肪分解、脂肪生成、脂肪酸（FA）氧化、ATP 含量、耗氧量、线粒体质量、柠檬酸合酶活性和线粒体氧化磷酸化（OXPHOS）复合物的蛋白质含量。
结果
在 9 天的时间里，用 16:1n7 处理可提高基础和异丙肾上腺素刺激的脂肪分解、FA 酯化、FA 氧化、耗氧量、OXPHOS 复合物 II、III 和 V 的亚基的蛋白表达以及细胞内 ATP 含量。用 16:0 处理的脂肪细胞则未观察到这些作用。
结论
棕榈油酸通过协同作用于脂肪分解、FA 酯化、线粒体 FA 氧化、耗氧量和 ATP 含量，可增强白色脂肪细胞的能量消耗，并可能作为局部激素发挥作用。
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