1 U.S. Food and Drug Administration, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, 1 Iberville Drive, Dauphin Island, Alabama 36528, USA; and.
2 RJ Hill Laboratories Ltd., Private Bag 3205, Hamilton 3240, New Zealand.
J Food Prot. 2018 Apr;81(4):677-683. doi: 10.4315/0362-028X.JFP-17-456.
The use of chloramphenicol (CAP) in aquaculture products is banned in many countries, including the United States, due to human health issues. Very few depletion and metabolism studies of CAP in seafood have been performed. Current detection methods for CAP residues in food are directed toward the parent drug molecule, but rapid elimination following treatment suggests the need for an alternative marker residue. We identified, characterized, and determined the persistence of two CAP metabolites, CAP-base (CAP-B) and CAP-alcohol (CAP-OH), in crab and shrimp. Interday recoveries of CAP, CAP-B, and CAP-OH in muscle fortified ( n = 9) at levels of 0.15 to 0.60 ng/g ranged from 95 to 127% and 101 to 119% for crab and shrimp, respectively, with repeatability ranging from 4 to 19%. The limit of detection for CAP and metabolites in crab and shrimp ranged from 0.05 to 0.11 ng/g. We also monitored the depletion of CAP, CAP-B, and CAP-OH in crab following waterborne exposures. To our knowledge, we present the first CAP depletion and metabolite study following waterborne exposure in crabs, with the aim of identifying alternative marker residues.
由于人类健康问题，许多国家（包括美国）已禁止在水产养殖产品中使用氯霉素（CAP）。目前，仅有少数关于 CAP 在海鲜中的消耗和代谢研究。目前食品中 CAP 残留的检测方法针对的是母体药物分子，但治疗后迅速消除表明需要替代的标记残留物。我们鉴定、表征并确定了两种 CAP 代谢物，CAP 碱（CAP-B）和 CAP 醇（CAP-OH）在蟹和虾中的持久性。肌肉强化（n = 9）中 0.15 至 0.60 ng/g 水平的 CAP、CAP-B 和 CAP-OH 的日内回收率分别为蟹和虾的 95%至 127%和 101%至 119%，重复性范围为 4%至 19%。蟹和虾中 CAP 和代谢物的检测限范围为 0.05 至 0.11 ng/g。我们还监测了水中暴露后蟹中 CAP、CAP-B 和 CAP-OH 的消耗情况。据我们所知，这是首次在蟹中进行的水相暴露后 CAP 消耗和代谢研究，旨在确定替代标记残留物。
