Zhang Junjun, Shen Haoyuan, Wang Mengjun, Nie Sheng, Wu Xizheng, Huang Yi, Gao Xiang
Department of Neurosurgery of Ningbo First Hospital, Ningbo University School of Medicine, Ningbo, Zhejiang 315010, China.
Department of Neurosurgery, Yuyao People's Hospital, Yuyao, Zhejiang 315000, China.
Curr Neurovasc Res. 2018;15(1):26-33. doi: 10.2174/1567202615666180319153628.
BACKGROUND: C-X-C motif chemokine ligand 12 (CXCL12) may play an important role in the development of Intracranial Aneurysm (IA). OBJECTIVE: The goal of this study was to explore the association between CXCL12 rs1746048 genotypes and circulating lipid concentrations along with the risk of IA. METHODS: A total of 256 IA patients and 361 healthy volunteers were included in the case-control study. The genotypes of CXCL12 rs1746048 were detected by Melting Temperature shift (Tmshift) Polymerase Chain Reaction (PCR). RESULTS: Significant higher levels were seen in Total Cholesterol (TC) (padjusted < 0.001), Highdensity Lipoprotein Cholesterol (HDL-C) (padjusted < 0.001), Low-density Lipoprotein Cholesterol (LDL-C) (padjusted < 0.001), Apolipoprotein A-I (ApoA-I) (padjusted = 0.040), and Apolipoprotein B (ApoB) (padjusted < 0.001) in IAs compared with controls. CXCL12 rs1746048 T allele frequency showed significant association with the risk of IA in the female group aged 65 or above (p = 0.019, Odds Ratio (OR) = 2.15, 95% confidence interval (95%CI) = 1.13 - 4.11, power = 64.8%). Moreover, CXCL12 rs1746048 was likely to be a risk variant of IA under the recessive model in females older than 65 years. (p = 0.030, OR = 3.77, 95%CI = 1.08 - 13.12, power = 81.8%). Additionally, we also found that the levels of LDL-C were significantly different among three genotypes (CC vs. CT vs. TT = 2.75±0.73 vs. 3.03±0.89 vs. 2.82±0.72, p = 0.035) in IA patients. CONCLUSION: Our results suggest that CXCL12 rs1746048 is significantly associated with IA risk in Han Chinese females aged 65 years and older. Additionally, the genotypes of CXCL12 rs1746048 may affect the LDL-C concentrations in IA patients.
背景：C-X-C基序趋化因子配体12（CXCL12）可能在颅内动脉瘤（IA）的发生发展中起重要作用。 目的：本研究旨在探讨CXCL12 rs1746048基因型与循环血脂浓度及IA风险之间的关联。 方法：病例对照研究共纳入256例IA患者和361名健康志愿者。采用熔解温度偏移（Tmshift）聚合酶链反应（PCR）检测CXCL12 rs1746048的基因型。 结果：与对照组相比，IA患者的总胆固醇（TC）（校正P<0.001）、高密度脂蛋白胆固醇（HDL-C）（校正P<0.001）、低密度脂蛋白胆固醇（LDL-C）（校正P<0.001）、载脂蛋白A-I（ApoA-I）（校正P = 0.040）和载脂蛋白B（ApoB）（校正P<0.001）水平显著升高。CXCL12 rs1746048的T等位基因频率与65岁及以上女性IA风险显著相关（P = 0.019，优势比（OR）= 2.15，95%置信区间（95%CI）= 1.13 - 4.11，检验效能= 64.8%）。此外，在65岁以上女性中，CXCL12 rs1746048在隐性模型下可能是IA的风险变异体（P = 0.030，OR = 3.77，95%CI = 1.08 - 13.12，检验效能= 81.8%）。此外，我们还发现IA患者三种基因型（CC vs. CT vs. TT = 2.75±0.73 vs. 3.03±0.89 vs. 2.82±0.72，P = 0.035）的LDL-C水平存在显著差异。 结论：我们的结果表明，CXCL12 rs1746048与65岁及以上汉族女性的IA风险显著相关。此外，CXCL12 rs1746048的基因型可能影响IA患者的LDL-C浓度。
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