Matsubara S, Tamada T, Saito T
Nihon Sanka Fujinka Gakkai Zasshi. 1987 Jul;39(7):1080-6.
Ca++ATPase is one of the most important regulatory systems that maintain the intracellular Ca++ concentration, which is essential to many cellular functions. The presence of Ca++ATPase in the human placenta has been speculated on biochemical grounds, but direct histochemical evidence has been lacking. Therefore, ultracytochemical localization of Ca++ATPase on trophoblastic cells of the human placenta was investigated by using the lead citrate method. Strong Ca++ATPase activity on the microvillous membrane and weak activity on the basal plasma membrane of the syncytiotrophoblast were observed. This distribution of Ca++ATPase was in good agreement with biochemical speculations. These results suggested that the syncytiotrophoblast might play an important role in the Ca++ metabolism in the human placenta.
钙离子ATP酶是维持细胞内钙离子浓度的最重要调节系统之一，而细胞内钙离子浓度对许多细胞功能至关重要。基于生化依据推测人胎盘中存在钙离子ATP酶，但一直缺乏直接的组织化学证据。因此，采用柠檬酸铅法对人胎盘滋养层细胞上的钙离子ATP酶进行了超微细胞化学定位研究。观察到合体滋养层细胞微绒毛膜上有较强的钙离子ATP酶活性，而基底质膜上活性较弱。钙离子ATP酶的这种分布与生化推测高度一致。这些结果表明，合体滋养层细胞可能在人胎盘的钙代谢中起重要作用。
