α-人心房利钠多肽对兔近端直小管容积重吸收和对氨基马尿酸分泌无影响。

Lack of effect of alpha-human atrial natriuretic polypeptide on volume reabsorption and p-aminohippuric acid secretion in the rabbit proximal straight tubule.

作者信息

Ando R, Sasaki S, Shiigai T, Takeuchi J

出版信息

Jpn J Physiol. 1987;37(1):81-91. doi: 10.2170/jjphysiol.37.81.

DOI:10.2170/jjphysiol.37.81

Abstract

To assess a possible direct tubular action of alpha-human atrial natriuretic peptide (alpha-hANP), we studied the effects of alpha-hANP on volume reabsorption (JV), transepithelial voltage (VT), and p-aminohippuric acid (PAH) secretion in the rabbit proximal straight tubules (PST) by in vitro microperfusion technique. In superficial PST (SFPST), addition of alpha-hANP at various concentrations (10(-7), 10(-9), 10(-11) M) to the bath solution did not alter JV significantly. Bath alpha-hANP (10(-7) M) did not change VT in SFPST, either. In juxtamedullary PST (JMPST), addition of alpha-hANP (10(-7) M) to the bath solution changed neither VT nor JV significantly. The alpha-hANP (10(-7) M) in the luminal fluid caused no changes in either VT or JV in SFPST. Furthermore, alpha-hANP (10(-7) M) in the bath did not change the rate of PAH secretion in SFPST. Thus, we could not obtain any evidence for direct tubular action of alpha-hANP in rabbit PST. Accordingly, alpha-hANP may increase solute excretion without directly affecting solutes transport in PST of the rabbit kidney.

摘要

为评估α-人心房利钠肽（α-hANP）可能的直接肾小管作用，我们采用体外微灌流技术研究了α-hANP对兔近端直小管（PST）中容积重吸收（JV）、跨上皮电压（VT）和对氨基马尿酸（PAH）分泌的影响。在浅表PST（SFPST）中，向浴液中添加不同浓度（10^(-7)、10^(-9)、10^(-11) M）的α-hANP对JV无显著影响。浴液中的α-hANP（10^(-7) M）对SFPST中的VT也无影响。在近髓PST（JMPST）中，向浴液中添加α-hANP（10^(-7) M）对VT和JV均无显著影响。管腔内液中的α-hANP（10^(-7) M）对SFPST中的VT或JV均无影响。此外，浴液中的α-hANP（10^(-7) M）对SFPST中PAH的分泌速率无影响。因此，我们未获得α-hANP在兔PST中有直接肾小管作用的任何证据。相应地，α-hANP可能在不直接影响兔肾PST中溶质转运的情况下增加溶质排泄。