Ogawa Y, Maeda T, Seguchi H, Yoshida S, Inomata T, Hamada F, Kishimoto S, Saito H, Osaki T
Oncology. 1987;44(4):219-23. doi: 10.1159/000226481.
Serial frozen sections were prepared from 22 squamous cell carcinomas of oral cavity and paranasal sinus. Mononuclear cell infiltrates were stained by the biotin-avidin-horseradish peroxidase method using a panel of 10 mouse monoclonal antibodies to human leukocyte antigens. The degree of infiltration was graded from + + + (marked) to - (absent). The infiltration of anti-Leu-4-reactive cells (Leu-4+ cells) was grade + + or + + + in 14 of 22 cases. In 13 of 22 cases, infiltration of Leu-3a + 3b+ cells (helper/inducer T lymphocytes) was grade + + (moderate). In 5 of 20 cases, infiltration of Leu-2a+ cells (cytotoxic/suppressor T lymphocytes) was grade + +. As for B lymphocytes, infiltration of Leu-12+ cells was grade + + in only 2 of 19 cases. In conclusion, T lymphocyte infiltrates were commonly seen in squamous cell carcinoma in oral cavity and paranasal sinus and the number of patients with grade + + infiltration of helper/inducer T lymphocytes significantly predominated over that of patients with infiltration of cytotoxic/suppressor T lymphocytes grade + +.
从22例口腔及鼻窦鳞状细胞癌制备连续冰冻切片。采用一组10种抗人白细胞抗原的小鼠单克隆抗体，通过生物素-抗生物素蛋白-辣根过氧化物酶法对单核细胞浸润进行染色。浸润程度从 +++（明显）到 -（无）分级。22例中有14例抗Leu-4反应性细胞（Leu-4+细胞）浸润为++或+++级。22例中有13例Leu-3a + 3b+细胞（辅助/诱导性T淋巴细胞）浸润为++级（中度）。20例中有5例Leu-2a+细胞（细胞毒性/抑制性T淋巴细胞）浸润为++级。至于B淋巴细胞，19例中只有2例Leu-12+细胞浸润为++级。总之，口腔及鼻窦鳞状细胞癌中常见T淋巴细胞浸润，辅助/诱导性T淋巴细胞浸润为++级的患者数量显著多于细胞毒性/抑制性T淋巴细胞浸润为++级的患者。
